The Sample Preparation Product Advisor recommends the most appropriate sample extraction device depending on the application requirements you need to consider. Click on the appropriate tab and move your mouse on the left hand side table to reveal the corresponding application in the middle of the screen.

• BioAnalysis, Foren...

  Solid-phase extraction (SPE) method development starts with knowing the sample matrix or application, the nature and relative concentration of the analytes of interest, and the potentially interfering endogenous compounds.  Then, judicious choices of sorbent and solvents for load, wash, and elution steps create rugged, reliable, and selective protocols.

• BioAnalysis

  The bioanalytical community faces unique challenges, such as: the need to maximize sensitivity, increase throughput, and develop new, robust methods that can be transferred to laboratories across the globe.

• Strong Acids/Aqueous Matr...

  An aqueous matrix solvent includes biological fluids such as: plasma, serum, urine, and tissue homogenates. Strong acids achieve complete ionization or dissociation in water.  If an analyte is 'strong' it will always be charged. An example of a strong acid includes decanesulfonic acid. Strong acids will have a lower pKa than weaker acids.

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  • Oasis WAX This product is the first choice when performing sample preparation of strong acidic compounds. The sorbent utilizes both ion exchange, for specific binding of strong acids, and reversed-phase mechanisms, for the retention of matrix.
  • Oasis HLB This product is preferred for general purpose reversed-phase clean-up, as most classes of compounds can be isolated reproducibly. An easy-to-follow protocol makes Oasis HLB an effective choice for sample preparation of compound mixes.
• Weak Acids in an Aqueous ...

  An aqueous matrix solvent includes biological fluids such as: plasma, serum, urine, and tissue homogenates. The majority of acids are weak acids. Acids do not dissociate completely in water. They are usually not charged at pH < 3 and are ionized at pH> 7. Weak acids will have a higher pKa value than strong acids.  Examples of weak acids include ketoprofen and salicylic acid.

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  • Oasis MAX This product is the first choice for sample preparation of acids. Oasis MAX uses a mixed- mode retention mechanism, both ion exchange and reversed phase. The anion exchanger allows specificity for weak acids while interferences bind by reversed phase.
  • Oasis HLB This product is preferred for general purpose reversed phase clean-up, as most classes of compounds can be isolated reproducibly. An easy to follow protocol makes Oasis HLB an effective choice for sample preparation of compound mixes.
• Strong Bases in an Aqueou...

  An aqueous matrix solvent includes biological fluids such as: plasma, serum, urine, and tissue homogenates. Strong bases achieve a complete dissociation in water and are always charged.  Strong bases include valethamate and other quaternary amines.

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  • Oasis WCX Oasis WCX provides the best sample preparation for strong bases and quaternary amines. The retention mechanism is mixed mode. Specifically, strong bases will retain via cation exchange while interferences are retained via reversed-phase mechanisms.
  • Oasis HLB This product is preferred for general purpose reversed phase clean-up, as most classes of compounds can be isolated reproducibly. An easy to follow protocol makes Oasis HLB an effective choice for sample preparation of compound mixes
• Weak Bases in an Aqueous ...

  An aqueous matrix solvent includes biological fluids such as plasma, serum, urine, and tissue homogenates. Weak bases achieve partial dissociation in water. Weak bases are generally positively charged at pH < 3 and are uncharged at pH > 12. Weak bases include codeine, methadone, and amitriptyline.

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  • Oasis MCX Oasis MCX provides the best sample preparation for bases. The retention mechanism is mixed mode. Specifically, bases will retain via cation exchange while interferences are retained via reversed phase mechanisms.
  • Oasis HLB This product is preferred for general purpose reversed-phase clean-up, as most classes of compounds can be isolated reproducibly. An easy-to-follow protocol makes Oasis HLB an effective choice for sample preparation of compound mixes.
• Neutrals/Aqueous Matrix

  An aqueous matrix solvent includes biological fluids such as: plasma, serum, urine, and tissue homogenates. Neutral compounds are uncharged or very weakly charged.&nbsp; Examples of neutral compounds include many steroids.

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  • Oasis HLB This product is preferred for general purpose reversed-phase clean-up of analytes not retained by ion-exchange mechanisms. An easy to follow protocol makes Oasis HLB an effective choice for sample preparation of neutral compounds.
  • Sep-Pak tC18 This silica-based Sep-Pak tC18 product is recommended for adsorbing drugs and their metabolites in serum, plasma or urine by strong hydrophobic interaction. It can be used for neutral compounds that retain too strongly on Oasis HLB.
  • Sep-Pak C8 This silica-based Sep-Pak product has moderate hydrophobicity and can be used for absorbing drugs and their metabolites or peptides in serum, plasma or urine by hydrophobic interaction.
• Peptide BioAnalysis

  The selective extraction of peptides from other endogenous biological components is one of the most challenging sample preparation tasks.  The use of highly selective mixed mode solid phase extraction can help ensure that you extract the peptide with high recovery and low matrix effects.

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  • Oasis Peptide µElution Method Development Plate A method development plate containing Oasis MAX (mixed mode anion exchanger) and Oasis WCX (mixed mode weak cation exchanger) along with a generic screening protocol. Mixed mode sorbents contain both reversed phase and ion exchange characteristics.
• Forensic & Toxicolog...

  Forensic toxicology labs are frequently required to perform broad screening techniques on complex biological samples to identify drugs of abuse and other toxicants. The utilization of different sample matrices in routine testing combined with the variety, number and concentration of potential compounds of interest in a sample can present a considerable challenge.

• Opioids

  The analysis of natural and synthetic opioid drugs continues to be an important aspect of forensic toxicology.  LC-MS/MS allows direct analysis of glucuronide metabolites without hydrolysis that is necessary with GC-MS techniques.  Solid phase extraction allows concentration of the analytes of interest, as well as the removal of matrix interferences leading to more accurate results.

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  • Oasis MCX for Opioids in Urine The use of the highly selective mixed-mode, strong cation exchanger (Oasis MCX) removes matrix components that can interfere with MS signals. Concentration of the polar glucuronides, in particular, improves accuracy in reporting of results.
  • Oasis MCX for Opioids, Stimulants and Benzodiazepines The use of the highly selective mixed-mode, strong cation exchanger (Oasis MCX) removes matrix components that can interfere with MS signals. Concentration of the analytes of interest also allows for more accurate quantitation and reporting of results.
• Cannabinoids

  Cannabinoids represent a growing challenge for law enforcement agencies and forensic laboratories. Finding new, fast and effective means of analyzing both natural and synthetic cannabinoids requires the successful extraction of a broad range of chemical properties from a variety of matrices.

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  • Ostro for Synthetic Cannabinoids in Urine The wide applicability of this sorbent and methodology makes this applicable to newly developed related compounds commonly found with synthetic cannabinoids, with minimal, if any, method modifications.
  • Oasis HLB for Synthetic Cannabinoids in Wole Bood Oasis HLB is ideal as a rugged, reversed-phase polymeric sorbent that will retain a wide range of related compounds commonly found in synthetic cannabinoids. It is water wettable and easy to use with a wide variety of matrices and solvents.
  • Oasis MAX for Natural Cannabinoids Oasis MAX is a highly selective, mixed-mode strong anion exchanger designed for the extraction of acidic compounds and metabolites from biological matrices. This makes it ideal for retaining the components of natural cannabinoids.

• POP & Pesticides

  Solid-phase extraction (SPE) method development starts with knowing the sample matrix or application, the nature and relative concentration of the analytes of interest, and the potentially interfering endogenous compounds.  Then, judicious choices of sorbent and solvents for load, wash, and elution steps create rugged, reliable, and selective protocols.

• Pesticides

  Residues are monitored in food and feed for safety issues in consumption. Pesticides are monitored in the environment mainly to ensure drinking water quality.

• Organochlorine / Organoph...

  These two classes of pesticide compounds are environmentally regulated by the EPA in soil and water. The monitoring these contaminants in food is also important.

  Many of these pesticides are illegal for current use and multi-residue screening method may not be the most suitable for the analysis of these compounds in food.

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  • DisQue dSPE Products Dispersive SPE comprised of Primary Secondary Amine, MgSO4, C18 and carbon black combinations to remove fatty acids, sugars, pigments etc from QuEChERS extracts prior to GC/MS or LC/MS analysis.
  • SepPak Florisil Florisil is an extremely polar sorbent that is a blend of magnesium oxide and silica gel. This highly active and weakly basic sorbent is used to retain low to moderate polarity pesticides from soil or water extracts prior to GC analysis. Florisil is commo
  • SepPak C18 This sorbent offers good retention for most pesticides in aqueous samples, and is not recommended for highly polar analytes. C18 is used for EPA method 525.
  • Oasis HLB This sorbent offers excellent retention for most pesticides in aqueous samples. It will retain pesticides that are too polar for C18 retention. Can also be used for clean-up of soil or tissue extracts after appropriate dilution with water.
  • SepPak PSA (Primary Secondary Amine) For sugar and fatty acid removal from QuEChERS extract prior to pesticide analysis by GC/MS or LC/MS analysis, PSA in cartridge or dispersive SPE format. Sugar is removed by polar interaction with primary secondary amine surface.
• Carbamate, Urea, Triazine...

  These three classes of pesticide compounds are environmentally regulated by the EPA in soil and water. The monitoring of these compounds is also important in food.

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  • DisQuE dSPE Products Dispersive SPE comprised of PSA, MgSO4, C18 and carbon black combinations to remove fatty acids, sugars, pigments etc from QuEChERS extracts prior to GC/MS or LC/MS analysis.
  • Oasis HLB This sorbent offers excellent retention for most pesticides in aqueous samples. Can also be used for clean-up of soil or tissue extracts after appropriate dilution with water.
  • SepPak PSA (Primary Secondary Amine) For sugar and fatty acid removal from QuEChERS extract prior to pesticide analysis by GC/MS or LC/MS analysis, PSA in cartridge or dispersive SPE format. Sugar is removed by polar interaction with primary secondary amine surface.
  • SepPak PS2 Polystyrene-divinylbenzene sorbent for general purpose pesticide analysis used in official Japanese methods.
• Nicotinamide

  A compound-specific method for this pesticide has garnered interest lately due to potential environmental impact within the bee population.

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  • DisQuE dSPE Products Dispersive SPE comprised of PSA, MgSO4, C18 and carbon black combinations to remove fatty acids, sugars, pigments etc from QuEChERS extracts prior to GC/MS or LC/MS analysis.
  • Oasis HLB This sorbent offers excellent retention for most pesticides in aqueous samples. Can also be used for clean-up of soil or tissue extracts after appropriate dilution with water.
  • SepPak PSA (Primary Secondary Amine) For sugar and fatty acid removal from QuEChERS extract prior to pesticide analysis by GC/MS or LC/MS analysis, PSA in cartridge or dispersive SPE format. Sugar is removed by polar interaction with primary secondary amine surface.
  • SepPak PS2 Polystyrene-divinylbenzene sorbent for general purpose pesticide analysis used in official Japanese methods.
• Phenoxyacid herbicides

  This class of pesticide compounds is environmentally regulated by the EPA in soil and water. The monitoring of this contaminant is also important in food.

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  • Oasis MAX This product is used for extraction of aqueous samples prior to GC or LC analysis for acidic pesticides. Can also be used for clean-up of soil or tissue extracts after appropriate dilution with water.
  • Oasis HLB This sorbent offers good retention for phenoxyacid herbicides and other acidic pesticides under acidic conditions. Can also be used for clean-up of soil or tissue extracts after appropriate dilution with water.
• Sulfonylurea

  Sulfonamides and Beta-lactams are commonly used as veterinary antibiotics

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  • DisQuE dSPE Products Dispersive SPE comprised of PSA, MgSO4, C18 and carbon black combinations to remove fatty acids, sugars, pigments etc from QuEChERS extracts prior to GC/MS or LC/MS analysis.
  • Oasis HLB This sorbent offers excellent retention for most pesticides in aqueous samples. It will retain pesticides that are too polar for C18 retention. Can also be used for clean-up of soil or tissue extracts after appropriate dilution with water.
  • SepPak PS2 Polystyrene-divinylbenzene sorbent for general purpose pesticide analysis used in official Japanese methods.
• Pesticides Multi-Residue

  Laboratories employ multi-residue methods to speed up results and meet regulatory requirements. These methods can be used for general screening or quantitation.

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  • DisQuE dSPE Products Dispersive SPE comprised of PSA (Primary Secondary Amine), MgSO4, C18 and carbon black combinations to remove fatty acids, sugars, pigments etc from QuEChERS extracts prior to GC/MS or LC/MS analysis.
  • SepPak C18 This sorbent is used in pass-through or dispersive mode (for retention of interferences, not for retention of analyte).
  • Oasis HLB This sorbent is excellent for multi-residue, multi-class extraction of pesticides from environmental waters samples. Can also be used for clean-up of soil or tissue extracts after appropriate dilution with water.
• Persistent Organic P...

  Persistent Organic Pollutants (POPs) are organic compounds that are resistant to environmental degradation through chemical, biological, and photolytic processes.  POPs have been observed to persist in the environment, to bioaccumulate in human tissue, animal tissue, and food chains. They can have a potentially significant impact on human health and the environment. Many of these compounds are endocrine disruptors.

• Multi-Class/ Multi-Residu...

  Labs employ multi class methods in order to speed up results and meet regulatory requirements. Can be used for general screening or quantitation.

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  • Oasis HLB For multi-class, multi-residue, Oasis HLB is the most universal reversed-phase sorbent.
• Brominated Flame Retardan...

  Brominated flame retardants (BFRs) are organobromide compounds that have an inhibitory effect on the ignition of combustible organic materials. Polybrominated diphenyl ethers (PBDEs) are the most common class of BFRs. The impact of BFRs on the environment and their potential risks for animals and humans is a growing concern. Food and water contaminated with flame retardants is a source of exposure.

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  • SepPak C18 C18 is the recommended sorbent for water and waste water analysis.
  • Oasis HLB An alternative choice to C18, which may be simpler and easier to use.
• Brominated Flame Retardan...

  Brominated flame retardants (BFRs) are organobromide compounds that have an inhibitory effect on the ignition of combustible organic materials. Polybrominated diphenyl ethers (PBDEs) are the most common class of BFRs. The impact of BFRs on the environment and their potential risks for animals and humans is a growing concern. Exposure to flame retardants occurs mainly through inhalation or ingestion of dust. Exposure from dermal contact with contaminated soil and dust may also occur.

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  • Sep-Pak Alumina Alumina is a highly surface-active polar sorbent with three varieties; acidic [A], neutral [N], and basic [B] sorbents. Acidic and basic alumina are also low-capacity ion exchangers in aqueous media, unaffected by high-energy radioactivity.
  • Sep-Pak Silica A polar stationary phase, used primarily to adsorb analytes from non-polar solvents like hydrocarbons, chloro- or fluoro-substituted hydrocarbons or less polar esters and ethers.
• PCBs in Soil and Tissues

  Fish consumption appears to be a major pathway of exposure. Soil contamination is also important because residues may leach into groundwater or surface water.

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  • SepPak Florisil Florisil is an extremely polar sorbent that is a blend of magnesium oxide and silica gel. This highly active and weakly basic sorbent is used to adsorb low to moderate polarity species from non-aqueous solutions.
  • SepPak Silica A polar stationary phase, used primarily to adsorb analytes from non-polar solvents like hydrocarbons, chloro- or fluoro-substituted hydrocarbons or less polar esters and ethers.
• PCBs in Water

  Polychlorinated biphenyls were formerly used in as hydraulic fluids, plasticizers, adhesives, fire retardants, and various other uses. Drinking water containing  PCBs can affect skin, thymus gland, immune, reproductive, or nervous systems, and may increase the risk of cancer.

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  • SepPak C18 C18 is the recommended sorbent for water and waste water analysis.
  • Oasis HLB An alternative choice to C18. Oasis HLB is simpler and easier to use; conditioning and equilibration steps can be minimized or eliminated.
• PFCs

  Perfluorinated chemicals (PFCs) are worldwide spread, persistent, toxic, and have a bioaccumulative potential. People are exposed through food, water, or from using commercial products. Some PFCs stay in the human body for many years.

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  • Oasis HLB For multi-class, multi-residue, HLB is most universal reverse phase sorbent. This includes many fluorinated acids and metabolites of 5 carbons or greater .
  • Oasis WAX Recommended for fluorinated acids (carboxillic and sulfonic, i.e., PFOS/ PFOA)
• Pharmaceuticals / Persona...

  Pharmaceuticals /Personal Care Products (PPCPs) include: prescription and over-the counter therapeutic drugs, veterinary drugs, fragrances, cosmetics, sun-screen products, diagnostic agents, and nutraceuticals.

  Sources of PPCPs are human activity, residues from pharmaceutical manufacturing, residues from hospitals, illicit drugs, veterinary drug use, especially antibiotics and steroids.

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  • Oasis HLB TThis product is preferred for general purpose clean-up, as most classes of compounds can be isolated reproducibly. A simple, easy to follow protocol makes Oasis HLB an effective choice for sample preparation of compound mixes.
  • Oasis WAX This product is the first choice when performing sample preparation of strong acidic compounds. The retention mechanism is mixed mode, both ion exchange and reversed phase. This improves retention and is specific for strong acidic compounds.
  • Oasis WCX Oasis WCX provides better sample preparation for strong bases and quaternary amines. The retention mechanism is mixed mode (both ion exchange and reversed phase), which improves retention for all types of basic analytes, especially strong bases.
  • Oasis MAX This product is the first choice for sample preparation of acids. Oasis MAX uses a mixed mode retention mechanism (both ion exchange and reversed phase), which improves retention and allows for specificity when working with acidic compounds.
• Phenolics

  Disinfectant phenolics may impact surface and groundwwater.

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  • Oasis HLB For multi-class, multi-residue, HLB is most universal reverse phase sorbent. This includes halogenated phenols.

• Veterinary Drugs a...

  Residues are monitored in food and feed for safety issues in consumption. Pesticides are monitored in the environment to ensure drinking water quality.

• Veterinary Drug Resi...

  Veterinary drug residues are monitored in food and feed for safety issues in consumption. These drugs are monitored in the environment mainly to ensure drinking water quality.

• Sulfonamides, Beta-lactam...

  Sulfonamides and Beta-lactams are common use  veterinary antibiotics.

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  • Oasis MAX Sulfonamides are very weak acids and a specific MAX method should be followed. Oasis MAX offers the best clean-up, unless the analytes are acid or base labile.
  • Oasis HLB If the compounds of interest are not stable at high or low pH, then HLB should be used; this is the best sorbent for reversed phase retention of polar drugs from food matrices. Otherwise, MAX will give better clean-up.
• Steroids

  Steroids are used as the main treatment for certain inflammatory conditions as well as growth regulation.

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  • SepPak C18 C18 is an excellent sorbent for these types of compounds due to the ability to retain compounds and elute them without the need of very strong solvents.
• Fluoroquinolones

  Fluoroquinolones are antibiotics that are commonly used in veterinary medicine to treat a variety of illnesses such as respiratory and urinary tract infections.

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  • Oasis MCX More ion-exchange capacity makes the MCX sorbent the first choice for these compounds. For highly complex matrices (i.e., chicken liver), both MCX and MAX sorbents may be required.
  • Oasis MAX More ion-exchange capacity makes the MCX sorbent first choice for these compounds. For highly complex matrices (i.e., chicken liver), both MCX and MAX sorbents may be required.
• Aminoglycosides

  Aminoglycosides are potent bactericidal antibiotic active against aerobic, gram-negative bacteria.

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  • Oasis HLB Use specific HLB method if a high degree of enrichment is not required (e.g., highly sensitive LCMS)
  • Oasis WCX Use WCX if significant enrichment is required for this family of compounds.
• NSAIDs

  NSAIDs are a class of drugs that provides analgesic (pain-killing) and antipyretic (fever-reducing) effects, and in higher doses, anti-inflammatory effects.

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  • Oasis MAX MAX sorbent best choice for acidic drugs in food and other biological matrices.
• Macrolides

  Macrolides are a class of naturally occurring antibiotics. Their action is primarily bacteriostatic.

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  • Oasis MCX Should be first choice for best clean-up, unless the analytes are acid or base labile.
  • Oasis HLB If the compounds of interest are not stable at high or low pH, then HLB should be used; this is the best sorbent for reversed phase retention of polar drugs from food matrices. Otherwise, MCX will give better clean-up and should be first choice.
• Tetracyclines

  The tetracyclines are a group of very broad-spectrum antibiotics. They are all structurally related.

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  • Oasis HLB If the compounds of interest are not stable at high or low pH, then HLB should be used; this is the best sorbent for reversed phase retention of polar drugs from food matrices. Otherwise, MAX will give better clean-up and should be first choice.
  • Oasis MAX Should be first choice for best clean-up, unless the analytes are acid or base labile.
• Miscellaneous

  To determine contaminant (i.e. melamine, explosives) levels/traces of diagnosis materials or synthesize drugs (radio isotopes), perform water removal, biomolecule analysis, or air quality control, a large variety of applications are carried out in the industry and medical field.

• Water Removal

  Removing residual water is critical to facilitate the evaporation process (if required), moreover any residual water in the final extract would be detrimental to good GC performance.

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  • SepPak Dry Waters Sep-Pak Dry cartridges are ideal for the removal of water from organic extracts prior to GC or GC/MS analysis. These cartridges are designed to remove residual water from extracts coming from solid phase extraction or liquid-liquid extraction.
• Air Quality / DNPH

  Air sampling for carbonele compounds for compliance with EPA and OSHA regulations.

   

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  • Sep-Pak DNPH This product has an acidified dinitrophenylhydrazine reagent coated silica sorbent. It is used to analyze air samples for aldehydes and ketones as their hydrazone derivatives.
  • Sep-Pak XPoSure This product has an acidified dinitrophenylhydrazine reagent coated silica sorbent. It is used to analyze air samples for aldehydes and ketones as their hydrazone derivatives.
  • Ozone Scrubber This product has a potassium iodide sorbent for removing ozone interferences. It is used in series with a Sep-Pak DNPH or an XPoSure Aldehyde Sampler cartridge.
• Explosives

  Testing for explosive analytes in environmental water samples, as per EPA method

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  • Sep-Pal PoraPak RDX Specially cleaned polymeric poly(divinylbenzene-vinylpyrrolidone) resin with hydrophobic character. Compatible with sample or eluents at high and low pH. Specifically designed for the concentration of high explosives in aqueous samples.
  • Oasis HLB Can be used as an alternative to PoraPak RDX. Recommended for explosive polar degradants.
• Melamine

  An industrial chemical to artificially inflate nitrogen content in foodstuffs, including pet food, infant formula, and milk/milk by-products. Matrix may include foodstuffs, tissue, urine, etc.

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  • Oasis MCX Recommended for melamine and basic degradants.
  • Oasis MAX Recommended for cyanuric acid and acidic degradants of melamine.
• Sugar

  Used for the removal of sugar from organic extracts such as QuEChERS.

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  • SepPak PSA (Primary Secondary Amine) For sugar and fatty acid removal from QuEChERS extract, PSA (Primary Secondary Amine) in cartridge or dispersive SPE format. Sugar is removed by polar interaction with primary secondary amine surface.
  • SepPak AminoPropyl Aminoproply is an alternative choice for the removal of sugar and fatty acid from QuEChERS extracts. Sugar is removed by polar interaction with amino groups. This method is slowly being replaced by PSA in regulated methods.
• Radio Isotope

  Many chemical elements have one or more radioactive isotopes, few are naturally occuring,  the rest are artificial. Radioactive isotopes have many useful applications (medicine, industry). In medicine, radioisotopes are increasingly  being used as a diagnosis tool (immaging). Tracers are radioactive isotopes introduced into the body to study metabolism or other biological processes.

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  • Sep-Pak Accell Plus QMA Sep-Pak Accell Plus QMA sorbent is silica-based, hydrophilic, strong anion-exchanger with large pore size (300Å). The sorbent is designed for extraction of anionic analytes in aqueous and non-aqueous solutions.
  • Sep-Pak Alumina Alumina is highly surface-active polar sorbent with three varieties; acidic [A], neutral [N], and basic [B] sorbents. Acidic and basic alumina are also low-capacity ion exchangers in aqueous media, unaffected by high-energy radioactivity.
  • Sep-Pak tC18 Silica-based bonded phase with strong hydrophobicity; trifunctional bonding chemistry gives it an increased hydrolytic stabilty; applications similar to C18.