The Bioseparations Columns Advisor, organized by biomolecule type, helps users select an appropriate UPLC/UHPLC or HPLC for a desired application. You can search by either Biomolecule / Separation Mode or by Column / Biomolecule with relevant offerings being highlighted.

Start by moving your mouse to the desired biocompound class in the left margin and click on the selectable tabs.  The middle screen will then reveal useful information to help you toward the select of a  recommended chemistry to address your need.

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• Peptides

  Chromatographic modes for peptide separation include reversed-phase, ion-exchange, hydrophilic-interaction, and size-exclusion chromatography and are used for applications ranging from the analysis and purification of synthetic peptides,  to the characterization of complex proteolytic digests, and bioanalysis. These methods rely on taking advantage of chemical and physical differences between peptides species.

• Reversed Phase

  Reversed-phase chromatography using ion-pairing reagent such as TFA and formic acid can deliver highly resolved separations of complex peptide mixtures whose sequences may differ by a single amino acid. In general, the hydrophobicity of the peptide determines the elution order, with the least hydrophobic peptides eluting first.

• HPLC and UPLC Columns

  Reversed-phase chromatography using ion-pairing reagent such as TFA and formic acid can deliver highly resolved separations of complex peptide mixtures whose sequences may differ by a single amino acid. In general, the hydrophobicity of the peptide determines the elution order, with the least hydrophobic peptides eluting first

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  • ACQUITY UPLC Peptide Columns Sub-2 micron UPLC Peptide columns are specifically designed for UPLC/UHPLC Systems to deliver high efficiency separation delivering superior component resolution and increased throughput compared to traditional HPLC.
  • XBridge Peptide Columns XBridge columns are BEH based C18 columns for the separation of a wide range of peptides and proteins.
  • XSelect Peptide Columns Xselect columns are charged surface hybrid (CSH) C18 reversed-phase columns for the separation of proteins and peptides. The CSH modification of these particles enables the use of more MS friendly mobile phases (e.g. formic acid).
• Nano Columns

  These peptide columns range from 75 µm to 1 mm I.D. These columns are well suited for high-sensitivity, sample-limited applications, and 2-dimensional chromatographic separations.

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  • ACQUITY UPLC M-Class Columns 15,000 PSI rated trapping and nano flow columns take advantage of the capabilities of the ACQUITY UPLC M-Class System to provide the highest peak capacities for the analysis of sample limited and two-dimensional separations of peptides.
  • nanoACQUITY UPLC Columns 10,000 PSI rated trapping and nano flow columns containing a variety of chemistries for use on Waters nanoACQUITY UPLC Systems for the analysis of sample limited and two-dimensional separations of peptides.
• Ion Exchange

  Ion-exchange chromatography separates peptides based on charge and is consequently used when a different / orthogonal separation mechanism to reversed-phase LC is desired.

• HPLC and UPLC Columns

  Ion-exchange chromatography separates peptides based on charge and is consequently used when a different / orthogonal separation mechanism to reversed-phase LC is desired.

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  • Protein-Pak Hi Res IEX Columns These columns are well suited for use with UPLC/UHPLC Systems. The non-porous, high binding capacity of these columns yields outstanding resolution of charged species in less time compared to traditional porous IEX columns.
  • BioSuite IEX Columns The methacrylic ester-based polymeric particle used in the BioSuite family of ion-exchange columns, contains either anionic or cationic ligands that can be used for the analysis and lab-scale purification of various peptides based on charge.
• HILIC

  Hydrophillic Interaction Chromatography (HILIC) is a useful alternative peptide separation technique to ion-exchange and reversed-phase LC.  This separation mode using Bridged Hybrid Technology (BEH) amide bonded stationary phases separates compounds based on their hydrophilicity. HILIC is ideally suited when attempting to separate glycosylated from non-glycosylated peptide containing mixtures.

• HPLC and UPLC Columns

  Hydrophillic Interaction Chromatography (HILIC) is a useful alternative peptide separation technique to ion-exchange and reversed-phase LC. This separation mode using Bridged Hybrid Technology (BEH) amide bonded stationary phases separates compounds based on their hydrophilicity. HILIC is ideally suited when attempting to separate glycosylated from non-glycosylated peptide containing mixtures.

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  • ACQUITY UPLC Glycan Columns These columns are QC tested for the HILIC separation of 2-AB labeled, released glycans and are suited for the separation of glycopeptides based on variation in the structure and occupancy of their glycan posttranslational modifications.
  • XBridge Glycan Columns These HPLC columns are specifically QC tested for the HILIC separation of 2-AB labeled, released glycans. They are also well suited for the analysis and lab-scale purification of glycopeptides.
• Size Exclusion

  Size exclusion chromatography, also known as gel filtration, separates molecules based on their hydrodynamic radii.   In general, a two-fold difference in the molecular weight of two different peptides is required to obtain baseline resolution of each species.

• UPLC and HPLC Columns

  Size exclusion chromatography, also known as gel filtration, separates molecules based on their hydrodynamic radii. In general, a two-fold difference in the molecular weight of two different peptides is required to obtain baseline resolution of each species.

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  • ACQUITY UPLC BEH SEC Columns These columns are designed and QC tested for use on a UPLC/UHPLC System. They yield higher resolving and increased sample throughput analyses of peptides, modified peptides and their aggregates as compared to traditional HPLC-based SEC analyses.
  • XBridge Protein BEH SEC Columns HPLC SEC columns are based on the same Waters ethylene bridged hybrid (BEH)-based particle technology with stable diol-bonding to deliver superior performance compared to traditional, 100% silica-based SEC offerings.
• Standards and Kits

  Waters line of peptide products offers options that help ease sample preparation burdens.  Products include protein digestion standards, complex peptide matrices and synthetic peptide standards for column benchmarking and system validation.

• Peptide Standards

  Waters line of peptide products offer specialized application relevant qualitative and quantitative standards that ease sample preparation burdens.  Products include complex digestion standards, unique peptide mixes and synthetic peptide standards.

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  • Digestion Standard Mixes Waters offers a line of qualitative digestion standards that include Enolase, BSA, Bovine Hemoglobin, Phos B, ADH, Ecoli and specialty mixes.
  • Quantitative Peptide Standards Highly purified labeled and unlabeled peptide standards are available for quantitative anaylses.
  • Qualitative Peptide Mixes The MassPREP Peptide Mix provides a complex mix of peptides great for troubleshooting chromatographic analysis.
  • Phosphopeptide Standards and Kits A range of purified phosphopeptide standards and mixes with complex digests are available along with a sample enrichment kit.
  • RapiGest SF Surfactant An anionic, enzyme and MS friendly surfactant used for protein digestions.

• Glycans

  The analysis of complex N and 0-linked glycans composed of frequently similar and repeating sugar moieties requires a highly discriminating chromatographic method. Hydrophilic-interaction liquid chromatography (HILIC) using a particle modified with an amide ligand can effectively separate a wide range of released unmodified or fluorescently labeled glycans.

• HILIC

  Hydrophilic liquid interaction chromatography (HILIC),  using Ethylene Bridged Hybrid Technology (BEH) amide bonded stationary phases, separates compounds based on their hydrophilicity This makes HILIC ideally suited for the LC and LCMS analysis of released glycans, and glycopeptides.

• HILIC Columns

  Hydrophilic liquid interaction chromatography (HILIC), using Ethylene Bridged Hybrid Technology (BEH) amide bonded stationary phases, separates compounds based on their hydrophilicity This makes HILIC ideally suited for the LC and LCMS analysis of released glycans, and glycopeptides.

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  • ACQUITY UPLC Glycan Columns These columns are specifically QC tested for the HILIC separation of 2-AB labeled, released glycans. It is also well suited for the peptide mapping of glycosylated from non-glycosylated peptide mixtures.
  • XBridge Glycan Columns These HPLC columns containing either 2.5 or 3.5 µm particles, were developed and are QC tested for the HILIC separation of released glycans. They may also be used for the analyses of glycopeptides based on their N-linked and O-linked glycosylation.
• Standards and Kits

  Waters glycan line offers labeled standards for column and system calibration and benchmarking, inclusive kits to ease the sample preparation burden and easy to use concentrated mobile phase buffers specific for glycan application work.

• Glycan Standards

  Waters glycan line offers labeled standards for column and system calibration and benchmarking, inclusive kits to ease the sample preparation burden and easy to use concentrated mobile phase buffers specific for glycan application work.

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  • Glycan Standards Both 2-AB and RapiFluor-MS™ labeled , released glycan standards are available for calibration, benchmarking column and system performance of ACQUITY UPLC or XBridge BEH Amide Columns.
  • GlycoWorks Sample Preparation The GlycoWorks sample preparation kits are available for 2-AB and RapiFluor-MS labeling of released N-glycans. These complete kits allow for ease of use and fast sample preparation with supreme sensitivity.

• Proteins

  Chromatographic modes for the analysis and purification of proteins  include reversed-phase, affinity, ion-exchange, hydrophobic interaction, and size-exclusion chromatography. These methods rely on taking advantage of chemical and physical differences between protein species.

• Reversed Phase

  
  Reversed-phase chromatography of proteins, done with columns consisting of wide-pore  size particles ( e.g, 300Å) functionalized with short-ligand length chemistries,  is a separation technique based on the relative hydrophobic characteristics of the proteins in solution. Gradients of increasing of organic solvent concentration are frequently used to affect separations in the presence of ion-pairing reagents that minimize undesired ionic interactions.

• HPLC & UPLC Columns

  Reversed-phase chromatography of proteins, done with columns consisting of wide-pore size particles ( e.g, 300Å) functionalized with short-ligand length chemistries, is a separation technique based on the relative hydrophobic characteristics of the proteins in solution. Gradients of increasing of organic solvent concentration are frequently used to affect separations in the presence of ion-pairing reagents that minimize undesired ionic interactions.

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  • ACQUITY UPLC Protein C4 Columns Sub-2 micron, 300Å pore size, high temperature stable UPLC protein columns deliver efficient separations while minimizing sample carryover. These columns contains BEH particles with C4 ligands and are QC tested with a protein mixture.
  • XBridge Protein C4 Columns These 300Å pore size protein columns are available in 3.5 µm, 5 µm, and 10 µm BEH particles containing C4 ligands. They are used for the isolation and lab-scale purification of proteins and deliver efficient separations while minimizing sample carryover
• HIC

  Hydrophobic Interaction Chromatography (HIC)  is characterized by the adsorption of compounds to a weakly hydrophobic surface at high salt concentrations, followed by elution with a decreasing salt gradient.  This technique is well suited for protein separations due its non-denaturing characteristic.

• HIC Columns

  Hydrophobic Interaction Chromatography (HIC) is characterized by the adsorption of compounds to a weakly hydrophobic surface at high salt concentrations, followed by elution with a decreasing salt gradient. This technique is well suited for protein separations due its non-denaturing characteristic.

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  • Protein-Pak Hi Res Columns Protein-Pak Hi Res HIC columns contain non-porous, polymethacrylate-based particles (2.5 μm)
• Ion Exchange

  Ion-exchange chromatography is a powerful technique with its ability to resolve different protein species, based on their surface charge. Separations are based on the use of either salt, pH, or salt/pH gradients.

• Ion Exchange Columns

  Ion-exchange chromatography is a powerful technique with its ability to resolve different protein species, based on their surface charge. Separations are based on the use of either salt, pH, or salt/pH gradients.

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  • Protein-Pak Hi Res Ion-Exchange Columns These columns are available in both cation-exchange (CM, SP) and anion-exchange (Q) chemistries. The non-porous, high binding capacity of this offering yields outstanding resolution of proteins in less time compared to traditional porous IEX offerings.
  • BioSuite Ion-Exchange Columns These columns are well suited for the HPLC analysis and lab scale purification of proteins in either anion (Q, DEAE) or cation-exchange (CM, SP) separation modes that provide the flexibility needed to addess various protein separations.
• Size Exclusion

  Size exclusion, also known as gel filtration, chromatography separates biomolecules based on their sizes (hydrodynamic radii) in solution.  This technique is frequently used to monitor aggregation of biotherapeutic proteins.

• HPLC & UPLC Columns

  Size exclusion, also known as gel filtration, chromatography separates biomolecules based on their sizes (hydrodynamic radii) in solution. This technique is frequently used to monitor aggregation of biotherapeutic proteins.

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  • ACQUITY UPLC Protein Columns SEC columns contain diol-coated, BEH Particles (125, 200 and 450Å) developed for use on low dispersion UHPLC systems to deliver industry leading resolution and high throughput separations. Batches are QC tested with protein standards to ensure consistency
  • XBridge Protein SEC Columns These SEC columns contain diol-coated, BEH Particles (125, 200 and 450Å) developed for use on HPLC systems to deliver high resolution protein / peptide separations. Batches are QC tested with relevant protein standards to help ensure consistency.
• Standards and Kits

  Waters protein standard line contains specially formulated and designed protein standard mixes to benchmark and monitor column performance and enhance and validate protein applications performed on UPLC, HPLC, or LC/MS.

• Protein Standards

  Waters protein standard line contains specially formulated and designed protein standard mixes to benchmark and monitor column performance and enhance and validate protein applications performed on UPLC, HPLC, or LC/MS.

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  • Complex Protein Mixes The MassPREP Protein Standard Mix is designed for verification of HPLC/UPLC insutuments and performance of columns used for protein analysis, specfically the ACQUITY UPLC and Xbridge BEH300 C4 columns.
  • IEX Anion and Cation Test Standards The IEX Anion and Cation Test Standards are unique recipes specially designed to be used on a regular basis for evaluation of column and system performance when using the Waters Protein-Pak Hi Res IEX Columns.
  • HIC Protein Standard Mix The HIC Protein Standard Mix is a unique recipe specially designed to be used on a regular basis for evaluation of column and system performance when using the Waters Protein-Pak Hi Res HIC column.
  • SEC Protein Standard Mixes The BEH125, 200 and 450 SEC Protein Standard Mixes are unique recipes specially designed to be used on a regular basis for evaluation of column and system performance when using the HPLC and ACQUITY UPLC SEC Columns.

• Oligonucleotides

  Chromatographic modes for oligonucleotide separations include reversed phase and ion exchange.  The applications include the analysis and purifications of synthetic oligonucleotides used in research, diagnostic reagents or biotherapeutic compounds.

• Reversed Phase

  The gradient separation of  synthetic oligonucleotides by ion-pair reversed-phase LC is based on sequence length with shorter failure sequence eluting prior to desired product.

• UPLC and HPLC Columns

  The gradient separation of  synthetic oligonucleotides by ion-pair reversed-phase LC is based on sequence length with shorter failure sequence eluting prior to desired product.

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  • ACQUITY UPLC Oligonucleotide Columns These columns frequently deliver N for N-1 resolution of complex detritylated oligonucleotide sequences due to the enhanced resolving power obtained using sub-2 μm particles, making this a viable option to electrophoresis based methods.
  • XBridge Oligonucleotide Columns These columns containing 2.5 µm particles are the preferred HPLC offering for detritylated oligonucleotide purifications. Increasing column length (plate number) will support higher sample loads or provide improved resolution.
• Ion Exchange

  HPLC-based ion-exchange chromatography, using a gradient of increasing salt concentration, can be effectively used to separate PCR products as well as plasmid forms based on differing charge density characteristics.

• Ion Exchange Columns

  HPLC-based ion-exchange chromatography, using a gradient of increasing salt concentration, can be effectively used to separate PCR products as well as plasmid forms based on differing charge density characteristics.

   

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  • GenPak FAX columns The GenPak FAX column consists of 2.5 µm non-porous resin particles functionalized with a weak anion-exchanger to provide outstanding resolution of synthetic DNA/RNA, PCR products, or plasmids.
• Standards and Kits

  Specially formulated, synthetic oligonucleotide standard designed for verification of HPLC/UPLC instrument and column performance for analysis of oligonucleotide applications.

• Oligonucleotide Standards

  The Oligonucleotide Standard line provides standards designed for verification of HPLC/UPLC instruments and performance of columns used for analysis of synthetic oligos. These specially formulated standards are unique to our column chemistry.

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  • MassPREP OST Standard This MassPREP Oligonucleotide standard is the same standard used for column QC and is recommended to be used on a regular basis for benchmarking column and system performance for analysis of analysis of synthetic oligonucleotides.

• Amino Acids

  Amino acid analyses include the separation and quantitations of either free amino acids or amino acids derived from protein hydrolysates. Matrix effects and accurate quantitations are some of the challenges that are adressed by pre-column derivatization and high resolution chromatographic separations.

• Reversed Phase

  The gradient separation of precolumn derivitizated amino acids by ion-pair, reversed-phase LC is based on the different hydrophobic characteristics of each labeled constituent.

• HPLC & UPLC Columns

  The gradient separation of precolumn derivitizated amino acids by ion-pair, reversed-phase LC is based on the different hydrophobic characteristics of each labeled constituent.

   

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  • AccQ-Tag Chemistry Kit Waters AccQ-Tag HPLC-based method uses, 6-aminoquinolyl-N-hydroxysuccinimidyl carbamate that derivatizes primary and secondary amines in a simple, single-step reaction to yield highly stable, fluorescent derivatives.
  • AccQ-Tag Ultra Chemistry Kit Based on AccQ-Tag HPLC AA derivatization method, AccQ-Tag Ultra offering is part of a complete solution that provides higher throughput.
  • Picot-Tag Columns TBA
• Standards and Kits

  Waters amino acid kits and reagents are designed for accurate and reproducible quantitation of amino acids from various sample matrices using either UPLC or HPLC methods.   

• Amino Acids Standards & K...

  Waters amino acid kits and reagents are designed for accurate and reproducible quantitation of amino acids from various sample matrices using either UPLC or HPLC methods.

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  • AccQ-Tag Ultra Amino Acid Kit Waters AccQ-Tag Ultra kit is a turnkey offering that enables accurate, reproducible quantitation of amino acids from various sample types and matrices using UPLC.
  • AccQ-Tag Amino Acid Kit Waters AccQ-Tag kit offers a qualified, rugged methodology for reproducible amino acid analyses using HPLC in a simple, single-step reaction to yield highly stable, fluorescent adducts.
  • Pico-Tag Amino Acid Kit Waters Pico-Tag method is a rugged methodology based on pre-column derivatization via the reaction of phenylisothiocyanate (PITC) and is designed for amino acid analysis using HPLC.