No sample preparation or chromatography required

MS-based analysis of complex sample matrices often requires sample preparation and chromatographic separation prior to the MS detection.  This approach can be time consuming and costly, particularly when large numbers of samples or replicates are required.  It can also be technically challenging and can introduce unwanted selectivity to the analysis.

The REIMS Research System with iKnife Sampling removes the need for sample preparation and chromatographic separation, allowing an accurate mass MS profile to be collected in the space of a just few seconds per sample.  This saves time and money, and makes it easier than ever before to obtain detailed molecular information from a range of sample types.

Furthermore, the long, flexible connection between the iKnife handheld sampling device and the MS allows greater freedom of movement than any other ionization technique, bringing the analysis to the sample.

Quickly determine differences within and between samples

With molecular profile data collected from multiple samples of similar type or origin, it is often not possible to determine significant differences by visual inspection, especially since the differences are often represented by changing ratios of multiple components rather than the presence or absence of a few key species.

Progenesis QI software offers an intuitive, workflow-based approach to process large data sets using powerful multivariate statistical methods.  This quickly highlights similarities and differences between samples, allowing them to be grouped in an objective and unbiased way.

Identify molecular markers responsible for sample differences

Once samples have been grouped according to their similarities and differences, it is important to understand the reasons for those differences in order to provide a more detailed understanding of the chemical or biological system under investigation. 

Progenesis QI highlights spectral features that cause the differentiation between sample groups, and then uses the accurate mass MS data to search databases and identify the molecular markers.  These identifications can then be confirmed for a greater level of confidence, for example by conducting targeted MS/MS experiments to obtain additional structural information.

Available on IMS MS/MS or MS/MS

Waters QTof systems provide the ideal platform for REIMS with iKnife sampling, with superior performance, proven robustness and reliability, and the flexibility to adapt to changing priorities and future needs. 

SYNAPT G2-Si HDMS

For the ultimate research capabilities

• Enhanced selectivity from unique ion mobility separation
• Versatile experimental options
• High performance accurate mass MS and MS/MS

Xevo G2-XS QTof

For accessible, routine sample analysis

• Maximum robustness with no compromise in performance
• Flexibility to adapt to changing needs
• Accessible to experts and non-experts alike

Potential to combine with UPLC/MS towards a holistic profiling approach

Both SYNAPT G2-Si HDMS and Xevo G2-XS QTof are compatible with a wide range of Waters chromatography inlets and ionization sources.  This allows aliquots of the same sample to be analyzed on a single mass spectrometry system using multiple different analytical techniques, for example REIMS, UPLC-MS and GC-MS, towards a holistic profiling approach.

Inlet Options

UPLC, Nanoflow UPLC, UPC², GC, APC

Ionization Options

ESI, nanoESI, ionKey/MS, APGC, APCI, ESCI, ASAP, DESI, MALDI*

*MALDI available on SYNAPT G2-Si HDMS only