ACQUITY UPLC M-Class System with HDX Technology
Quantify changes in protein conformation with confidence
Monitoring protein conformation can be challenging. With the ACQUITY UPLC M-Class System with HDX Technology, your lab can leverage UPLC separations and high-resolution MS (HRMS) to answer important questions about protein conformation through the power of hydrogen deuterium exchange (HDX), like drug candidate binding to a protein, biopharmaceutical product development, protein-protein interactions, and intellectual property.
The system provides HRMS and intuitive informatics for confident measurement of changes in protein conformation by controlling important experimental parameters. With the ACQUITY UPLC M-Class System with HDX Technology, your lab can confidently identify changes in protein conformation by stringently controlling the labeling reaction, and efficiently processing and separating the sample before mass analysis.
ACQUITY UPLC M-Class System with HDX Technology
Specifications
|
Maximum operating pressure |
15,000 psi |
|
pH range |
2 to 10 |
|
Operating flow rate range |
200 nL/min to 100 μL/min without flow splitting |
|
Sample flow path |
Non-ferrous |
|
Injection volume |
50 µL loop standard; 1, 2, 5, 10, 20, 100, 250 µL loops available |
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Sample compartment |
0–25 °C |
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Pump options |
Binary |
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Detector options |
PDA Detector Mass Spectrometers |
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External control |
MassLynx Software |
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Physical specifications |
Width: 34 cm (14 in.) Height: 76 cm (30 in.) Depth: 71 cm (28.0 in.) |
Overview
- Rapid chromatographic resolution at 0 °C with UPLC technology
- Temperature-controlled environment for trapping and analytical columns
- Repeatable peptic digestion with Waters Enzymate BEH Pepsin Column, capable of operation up to 15,000 psi
- Easy access injection port for flexibility with manual or robotics injection
- Integrated with Waters high resolution MS for precise determination of Deuterium label
- Intuitive, interactive DynamX HDX Data Analysis Software to shorten the time between experiment and answers
Recommended Use: Answering important questions about changes in protein conformation.
Features Header
Accurately, reproducibly, and easily perform protein conformation studies
The unprecedented system-level design integrates all the steps necessary to accurately, reproducibly, and most of all, easily, perform protein conformation studies. The system and its DynamX HDX Analysis Software remove the barriers to adopting high resolution LC and MS for routine studies of protein conformational changes, making hydrogen deuterium exchange with MS (HDX, HDX MS, HXMS, or H/D exchange) a core competency for your laboratory.
Higher efficiency separations
Carefully controlling temperature-sensitive parts of the HDX workflow ensures that the conformational changes are captured and not missed. UPLC separations of up to 15,000 psi take place within the cooled environment with the added advantage of sub-2-µm particles for enhanced chromatographic resolution and speed. Maintaining temperatures at 0±1 °C means that back-exchange is kept to an absolute minimum with the ACQUITY UPLC M-Class System with HDX Technology.
Once the HDX reaction has been chemically quenched and manually introduced, the HDX Manager directs the sample to the pepsin column for peptic digestion, or directly into the mass spectrometer (MS) for global analysis. The sample is then trapped for a rapid UPLC analytical separation.
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