Analysis of Hydrolysate and Cell Culture Standards

The upper trace shows the analysis of a hydrolysate standard at 10 pmol/mL, and the lower trace a standard mixture of the larger number of amino acids found in cell culture media, also at 10 pmol/mL. The adaptation of the method requires only a change in dilution of the Eluent A Concentrate supplied as part of the total system solution.

Direct Cell Culture Sample Analysis

Aliquots of cell culture media were taken directly from a bioreactor producing a biopharmaceutical protein. Samples were taken over a period of six days and no special sample preparation was required before the amino acid analysis shown here. The amino acid concentration trends can be plotted with growth as well as the response to feeding.

Analysis of a Hydrolysate Standard at 10 pmol/mL

The UPLC Amino Acid Analysis Solution provides high resolution to ensure method robustness with good quantitation. The short cycle time is obtained without compromising accurate results.

Efficient, Reliable Foods and Feed Sample Analysis: Soybean Meal Hydrolysate

Two samples of hydrolyzed soybean meal were analyzed. The upper trace shows the untreated sample while the lower trace shows the results of performic acid oxidation. Note the appearance of cysteic acid and methionine sulfone in the oxidized sample.

Direct Cell Culture Sample Analysis

Aliquots of cell culture media were taken directly from a bioreactor producing a biopharmaceutical protein. Samples were taken over a period of six days and no special sample preparation was required before the amino acid analysis shown here. The amino acid concentration trends can be plotted with growth as well as the response to feeding.