How RapiGest Works

RapiGest SF is a mild denaturant that solubilizes and unfolds the substrate protein, making it more amenable to cleavage.  Mild protein denaturation opens the protein structure and exposes the proteolytic sites to enzymatic cleavage.  More complex denaturation of globular proteins can be accomplished by heating in a RapiGest SF solution at elevated temperatures.

Compatibility with MS

RapiGest SF is an acid labile surfactant that undergoes hydrolysis in acidic conditions.  This unique feature can be utilized to remove the surfactant from solutions when desired.  Acidic conditions break down RapiGest SF to non-interfering by-products, therefore peptide ionization is not suppressed.

The RapiGest SF Advantage

RapiGest SF shows significant advantage over other denaturants since it is not disruptive to endoprotease activity.  It allows use of lesser amounts of expensive endoproteases.  It is effective in a range of digestions such as Asp-N, Glu-C, Lys-C, PNGaseF and chymotrypsin digestions with the added advantage of significantly decreasing proteolytic digestion time, enabling same day digestions and analysis.

• Increases peptide recovery and coverage
• Facilitates the digestion of proteolytically resistant proteins
• Increases solubility of hydrophobic proteins/peptides