Reproducible

• Optimized kit-based approach delivers mean %CVs of < 6% using multiple peptides for 4 representative mAbs in plasma
• Streamlined protocol reduces the number of sample preparation steps
• Inter-kit and intra-kit precision ensures reproducible results, day to day, lab to lab, and analyst to analyst

     Download the Application Note

Sensitive

• High sensitivity detection limit of 10 ng/ml was achieved for infliximab in rat plasma
• Kits are optimized for the digestion of whole plasma or immunopurified samples
• uElution SPE improves assay sensitivity through sample concentration and the removal of salts, phospholipids, and residual digestion reagents

     Download the Application Note

Standardized & Simplified

• Broadly applicable protocol eliminates Method Development for Discovery Studies
• Kit-based approach is easily deployable from lab to lab, site to site, and sponsor to CRO
• Simple, well-defined protocols and pre-measured lot-traceable reagents facilitate method transfer and provide a well-researched starting point for those new to protein bioanalysis

For Post-Digestion Sample Clean-Up, use the ProteinWorks uElution SPE Kit

 (sold separately or included in ProteinWorks eXpress Digest and eXpress Direct Digest Start-up Kits) 

Optional intact murine mAb standard is ideal for workflow training and system health checks

 (sold separately or included in ProteinWorks eXpress Digest and eXpress Direct Digest Start-up Kits)