Waters invests significant research and development time to deliver solutions that help organizations address today's separation challenges. As a primary manufacturer of separations media, our ability to consistently deliver reliable, quality products, and support is second to none in the industry. Discover how our BEH300 C₄ columns supported by our world-class R&D, applications, and distribution organizations, help make the "Science of What's Possible" a reality.

• Well characterized, state-of-the-art bonding procedures
• Low pH and high temperature stability
• Quality-control tested with diverse protein mixture
• Consistent batch-to-batch reproducibility

BEH300 C₄ vs. Competitive Column Stability Under Protein Separation Conditions

Overlay of 6 injections on XBridge^TM BEH300 C₄ vs. competitive 100% silica, 300Å C4 column. Column stored with 0.1% TFA at 90 °C between protein sample injections at times 0, 5, 10, 40 and 60 hours.

Rigorous Manufacturing and Testing Procedures Help Ensure Consistent Batch-to-Batch Performance

Outstanding Batch-to-Batch Performance Consistency when Tested with a Complex Protein Test Mixture.

In 2006, Waters introduced UPLC^® Technology that demonstrated outstanding performance enhancements in resolution and speed compared to use of traditional HPLC. Our new BEH300 C₄ material is available in two particle-sizes for easy transfer an HPLC protein separation method using 3.5 µm particles to the benefits of ACQUITY UPLC^® Technology using 1.7µm particles. In addition, BEH300 C₄ material can be effectively run with mass spectrometry compatible eluents for collection of the information rich data possible using this advanced detection technique.

• Easily scaled 3.5 μm HPLC to 1.7 μm UPLC for enhanced separations
• Effective with MS compatible eluents to address the need for advanced detection techniques

                    1.7 μm Particle Technology Benefits to Complex Protein Separations

Use of 1.7 μm particle technology yields improved resolution within mAb heavy chain fragment. Note that the full performance benefits of the ACQUITY^® BEH300 C₄, 1.7 μm column will only be realized with the low system volume and low detector dispersion of an optimized ACQUITY UPLC system.

Compared to many 100% silica-based reversed-phase columns, BEH300 C₄ offerings can be successfully used with MS friendly eluents.