Accuracy: Quantitative Peptide Standards

When accuracy is critical, Waters offers several quantitative peptide standard mixes specifically designed to be run before your analysis to ensure LC or LC-MS system confidence.  They can also be spiked into complex samples to help with analytical accuracy.  The Quantitative Peptide Retention Standard is useful during calibration, development, and troubleshooting of chromatographic separations, ensuring confidence in results.  In addition, the Hi3 line of Peptide Standards provides MS users with a purified sample mix of the 6 top ionizable peptides in Phosphorylase B and E.Coli.

Benchmarking: Reversed-Phase Peptide Standards

The ability to qualify and troubleshoot your bioseparation with a standard that is similar to your samples is critical.  The Cytochrome C Digestion Standard is part of a line of qualitative digestion standards used for performance evaluation of HPLC and UPLC columns for peptide separation.  This standard is used to benchmark the ACQUITY UPLC and XBridge BEH C₁₈ 300Å and 130Å as well as the ACQUITY UPLC CSH and XSelect Column lines.  In addition, the MassPREP Peptide Mixture is a complex mix of nine carefully selected peptides with a broad range of polarities and isoelectric points used for the evaluation and troubleshooting of HPLC and UPLC columns and systems dedicated for peptide separations.

Dig Deeper: Phosphopeptide Enrichment and Standards

Phosphorylation is a prevalent post-translational modification (PTM) for regulating protein function.  The MassPREP Phosphopeptide Enrichment Kit provides selective enrichment of phosphopeptides from complex samples through affinity based SPE.  In addition, there is a suite of standards,  ranging from simple to complex, that consist of highly purified sample sets of synthetic phosphopeptides used to optimize phosphopeptide detection in LC-MS, LC-UV, and MALDI-MS analyses.

Complexity Made Simple: Digestion Standards

Protein digestions can be very time consuming and irreproducible.  Waters offers a variety of protein digests valuable to use as complex biological matrices for proteomic evaluations as well as for performance evaluation of HPLC, UPLC columns and LC-MS instrumentation.  They are especially useful in positive ion mode evaluations for sensitivity, resolution, and mass measurement accuracy on nanoAcquity and QToF based systems. 

Biopharma Relevant: mAb Digestion Standards

Peptide level analysis of proteins by LC-MS is one of the primary approaches for the characterization of biopharmaceuticals, in particular monoclonal antibodies (mAbs). In such an analysis, it is possible to acquire peptide mass information, quickly confirm protein sequence and post-translation modifications, as well as degradation related critical quality attributes. To support this application area, Waters has commercialized several digestion standards, which can be used in the benchmarking of LC-MS techniques, proficiency testing among different instruments and laboratories, and system suitability when performing peptide level protein analysis.

Available:

• mAb Tryptic Digest Standard*
• mAb Subunit Standard*
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 *These standards are stabilized and lyophilized format of a reduced and alkylated tryptic digestor the IdeS-digested NIST Reference Material 8671 (NIST mAb), a humanized IgG1κ expressed from a murine cell line. Disclaimer: Reference values for RM 8671 are not certified to be in correspondence with any Report of Investigation offered by the National Institute of Standards and Technology (NIST). If it is desirable to have such correspondence, it is advised that RM 8671 be directly acquired from NIST (nist.gov).