The ability to reproducibly generate high quality peptide separations is of critical importance in proteomics research as well as for the characterization of biopharmaceuticals via peptide mapping.  Reversed-phase (RP) chromatography has become the separation mode of choice due to its relatively high resolving power and outstanding quantitative (UV) and qualitative (ESI-MS) information.  Waters Ethylene Bridged Hybrid (BEH Technology) and new Charged Surface Hybrid (CSH™) Peptide Separation Technology columns provide capabilities that help address demanding application needs.

NEW ACQUITY UPLC and XSelect CSH130 C₁₈ Guards and Columns

• Yields superior peak capacity than many alternative C₁₈ column offerings.
• Best offering for LC/MS applications using 0.1% formic acid.
• Delivers less peptide retentivity than BEH-based C₁₈ offerings, but is suited for separations of polypeptides up to 10,000 Daltons.
• Scalable 1.7 µm, 3.5 µm, and 5 µm particles that address a variety of application needs.
• Tested with tryptic digests of cytochrome c for consistent batch-to-batch and column-to-column consistency.

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ACQUITY UPLC and XBridge BEH130 and BEH300 C₁₈ Guards and Columns

• Ideally suited for the separation of wide range peptides: large and small, acidic and basic, hydrophilic  and hydrophobic.
• 130Å and 300Å pore offerings for different separation selectivities and for use with polypeptides less than or greater than 10,000 Daltons.
• Scalable 1.7 µm, 3.5 µm, and 5 µm particles that address a variety of application needs.
• Specifically QC tested with tryptic digests of cytochrome c that helps ensure batch-to-batch consistency in validated methods.