ACQUITY UPLC Oligonucleotide C₁₈, 1.7 μm (designed for use with an ACQUITY UPLC System) and XBridge Oligonucleotide C₁₈, 2.5 μm Columns are well suited for the analysis of detritylated oligonucleotides using ion-pair, reversed-phase chromatography.

Oligonucleotide columns packed with BEH Technology particles have shown remarkable column longevity, under these demanding separation conditions, while maintaining outstanding separation performance.

Ion-pairing reversed phase (IP-RP) liquid chromatography is a commonly accepted separation strategy for the analysis and purification of detritylated oligonucleotides. The ion-pairing additive in the mobile phase is adsorbed on a hydrophobic C18 sorbent and provides for charge-to-charge interactions with negative charges contained on the oligonucleotide backbone (i.e., phosphate groups).

As a result, an efficient charge-based (length-based) oligonucleotide separation is achieved. Gradient elution using an acetonitrile or methanol eluent displaces both ion-pairing agent and the oligo­nucleotides from the sorbent surface while separation selectivity and resolution decreases with increasing oligonucleotide length making the separation of long oligonucleotides challenging.

The pre-packaged MassPREP Oligonucleotide Standard is designed for verification of HPLC/UPLC instruments and column performance for analysis of synthetic oligonucleotides.

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Desalting of synthetic oligonucleotides is essential for MS analysis (QC, genotyping applications and SNP analysis). The Oasis μElution Plate combines patented plate design, proven Oasis chemistries, and generic protocols enabling elution volumes as low as 25 μL. You can perform SPE clean-up and concentration of very small sample volumes. The Oasis Hydrophilic-lipophilic-balanced (HLB) sample extraction products incorporate a patented copolymer made from a balanced ratio of two monomers; the lipophilic divinylbenzene and the hydrophilic N-vinylpyrolodone.

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