The analysis of oligonucleotides by LC coupled with mass spectrometry is becoming a common practice. Many applications require the identification and analysis of oligonucleotides, modifications, variants, and process-related impurities at extremely low concentrations. With versatile configurations that can match your application requirements, Waters' portfolio of MS technologies provides the most appropriate solution for your laboratory's needs.
ACQUITY SQD
The ACQUITY SQD combines the resolution, sensitivity, and speed of UPLC technology with single quadrupole MS detection. Designed specifically for chromatographers performing UPLC/MS analysis, it offers a simple yet powerful solution for multi-tasking, fast-paced, or routine laboratory environments - with robust and reliable performance, and walk-up operation for any expertise level.
The ACQUITY UPLC System's ability to produce unmatched resolution, combined with the spectral quality available with the ACQUITY SQD, enables easy identification of compounds in applications, including:
- Peak purity and impurity profiling
- Synthesis confirmation
- Product deformulation
LCT Premier XE
The LCT Premier XE benchtop orthogonal acceleration time-of- flight (oa-TOF) mass spectrometer delivers high sensitivity, resolution, and exact mass measurements for LC/MS oligonucleotide analyses. Its fast data acquisition rates and automated workflow features match the requirements for MS detection under UPLC conditions, including:
- High MS resolution for the selectivity needed to separate analyte spectra from isobaric interferences and background chemical noise
- High sensitivity for achieving very low detection limits
- High linear dynamic range, which allows experiments to be carried out across a range of concentration levels
- Exact mass MS measurements, which give elemental composition information that can be used to identify analyte compounds; you can obtain exact mass on molecular and fragment ions, simplifying the process of spectrum interpretation
Q-Tof Premier
The Q-Tof Premier, a quadrupole, orthogonal acceleration time-of- flight (oa-TOF) tandem mass spectrometer, provides enhanced levels of flexibility, sensitivity, specificity, and speed of MS data acquisition. It enables automated exact mass measurement of precursor and fragment ions to yield the highest confidence in structural elucidation and databank search results. Featuring T-Wave (Travelling Wave) Technology, the Q-Tof Premier offers significant enhancements in mass measurement accuracy, dynamic range, sensitivity with Enhanced Duty Cycle, and speed.
Highlighted application work:
UPLC/MS Analysis of Interfering RNA Oligonucleotides
