UPLC HILIC/TUV/MS is suitable for characterization of glycoproteins and in particular for monoclonal antibodies, which represent the main class of biotherapeutics. This method can speed up the development and quality control of new therapies, as well as improve safety and efficacy of protein drugs.

• The ACQUITY UPLC BEH HILIC Glycan Column facilitates good resolution of glycopeptides from non-glycosylated peptides.
• Peptide glycoforms are well resolved for mAb related glycans.
• The method eliminates the need for glycan fluorescent labeling and SPE purification, and is complementary to established glycan characterization methods.
• Mobile phase permits sensitive MS detection.
• MS detection is helpful for site occupancy analysis (glycan micro-heterogeneity) of glycoproteins with multiple glycosylation sites (complex glycopeptides).
• The HILIC method complements RP peptide mapping, which does not provide sufficient information on glycan heterogeneity.