The HILIC Advantage

Hydrophilic Interaction Chromatography (HILIC) can be used to successfully improve retention of very polar species.  This technique is rapidly growing for this application area.  One reason for this is that this mode of separation is well suited for glycans due to their highly polar nature.  The GlycoWorks HILIC SPE provides an efficient and reproducible mechanism for effective glycan cleanup and provides quantitative and consistent recoveries for a diverse range of N-Glycans.

Inclusive Methodology

The GlycoWorks Kit offers a protocol for complete sample processing of released N-glycans with either 2-AB or 2-AA labeling.  Two formats offered, one for high-throughput which is a 96-well plate and the other for low volume users in a 1 cc cartridge format. 

Kit components include:

• Sample cleanup devices with HILIC (amino-propyle) sorbent
  • High-throughput: GlycoWorks HILIC µElution SPE Plate (96-well)
  • Single Use: GlycoWorks HILIC 1 cc (20 pack) and GlycoWorks HILIC 1 cc Flangeless Cartridges for the positive pressure manifold (20 pack)
• GlycoWorks Control Standard (un-labeled Human IgG) for method development/troubleshooting
• GlycoWorks Reagent Kit with material for protein deglycosylation and labeling