Column Features

Waters Enzymate BEH Pepsin Column:

• Provides fast, reproducible, and efficient online protein digestion, typically within 30 seconds
• Shortens overall protein sample preparation time
• Offer the ability to optimize protein digestion efficiency by changing temperature and/or flow rate

Low and High Pressure Digestion Efficiency Comparisons

Shown on the right is the digestion efficiency comparison between low and high pressure digestions. The Waters Enzymate BEH Pepsin Column was used for digestion pressure at 1,000 psi (low) and 13,500 psi (high). Higher pressure digestion enhanced digestion efficiency.

ACQUITY UPLC M-Class HDX System

Hydrogen deuterium exchange mass spectrometry (HDX MS) is used to study a protein’s structural dynamics and conformational changes, a component of understanding a protein’s higher order structure. Information about protein conformation from an HDX MS study can be used to compare a control with an analyte by measuring the relative amount of deuteration uptake. HDX MS is capable of monitoring domain interaction, localized protein breathing, and folding or unfolding in the solution phase. The ACQUITY UPLC M-Class System can quantify small changes in protein conformation by extending its pressure range to enable a higher efficiency separation. An integral part of the ACQUITY UPLC M-Class HDX System is the Waters Enzymate BEH Pepsin Column which performs online protein digestion.