AccQ•Tag Ultra and AccQ•Tag Derivitization Chemistry

This line of products is a comprehensive, fully tested set of reagents, columns, eluents and methods.   

AccQ•Tag is optimized for use with HPLC/UHPLC

AccQ•Tag Ultra is optimized for use with the UPLC Amino Acid Analysis Application Solution

• Both methods are based on the AccQ•Tag pre-column derivatization method
• Primary and secondary amino acids are reacted with a single reagent (AccQ•Fluor) in a high-throughput batch process resulting in exceptionally stable derivatives
• AccQ•Tag Ultra  provides high-resolution separations achieved using the AccQ•Tag Ultra UPLC column (1.7 µm particles) and mobile phases
• Derivatized amino acids resolved and quantified to sub-pmol levels with single wavelength UV detection

Application: Protein Characterization and Cell Culture

Whether you need to determine amino acid composition of a product and compare it to the gene sequence or it's natural counterpart or if you are downstream and monitoring cell culture media for critical nutrients to optimize expression efficiencies and make timely product harvesting decisions, the UPLC Amino Acid Analysis solutions provide one solution for rugged and reliable performance to help make correct decisions about the product throughout an organization.

• Confirm quality
• Demonstrate process consistency
• Meet production schedules to minimize costly delays

Application: Food and Feeds

Animal feeds pose several significant challenges for accurate amino acid analysis. Quantitation of amino acids released by hydrolysis is one important measure of nutritional value. A free amino acid profile can identify the origin of a food product, and in that way, detect adulteration. Free amino acids are also metabolic indicators that can be used to monitor and optimize processes such as fermentation

• Avoid costly reformulations
• Ensure products meet label claims for nutritional composition
• Confidence that products meet the manufacturing specifications

Turnkey Solution

The UPLC Amino Acid Analysis is a total application solution optimized for accurate, reliable, and reproducible analysis of amino acids. Based on AccQ•Tag Ultra chemistry combined with Waters award-winning ACQUITY UPLC separation technology, the UPLC Amino Acid Analysis Application Solution is designed for protein characterization, cell culture monitoring, and the nutritional analysis of foods and feeds.

Features:

• ACQUITY UPLC System and tunable UV detector
• AccQ•Tag Ultra derivatization chemistries including column, reagents, and eluents
• Empower 2 pre-configured projects, methods, and report templates
• Full system and application-level support documentation
• Application-specific Performance Qualification
• Connections INSIGHT

Pico•Tag Derivitization Chemistry for HPLC

 The Pico•Tag method is applicable to many samples including protein hydrolysates, physiologic fluids, feeds, foods, and pharmaceutical preparations. Pre-column derivatization relies on the coupling reaction of the well-known Edman Degradation, the reaction of phenylisothiocyanate (PITC) with both primary and secondary amino acids to form phenylthiocarbamyl (PTC) derivatives The PTC-amino acid adducts are stable and easily separated by reversed-phase HPLC. A single product is formed for each amino acid.

• The Pico•Tag Amino Acid Analysis Column is a high-efficiency column specifically certified for use with the Pico•Tag method. This HPLC column separates the amino acid derivatives produced by phenylisothiocyanate (PITC) derivitization.
• The Pico•Tag workstation is required to perform the Pico•Tag methodology. The workstation and parts can be purchased through Eldex by clicking the following link:
• Pico•Tag workstation