Symmetry300是基于Symmetry^®高纯硅胶基质、但颗粒孔径为300Å的反相色谱柱。由高纯有机试剂起始进行合成所得的高纯硅胶，硅醇基活性很低，有利于获得对称的峰形。Symmetry300 HPLC分析柱是沃特世首个为生物制药所需而专门设计的色谱柱产品。

• 适用于多肽、蛋白质分析
• 峰形出色（UV和MS）
• 峰容量高

此款色谱柱有两种型号供选择：

孔径效应对肽分离选择性的影响：Symmetry 100Å（上图） Vs. Symmetry300(下图)

蛋白质分离结果：Symmetry300 C4 Vs. 其它品牌色谱柱

Symmetry^® Columns are synonymous with reproducible chromatographic results. 
Narrow column specifications help you to achieve reproducible results. The surface coverage requirements of Symmetry Columns are the tightest specifications in the industry, resulting in minimal shifts in resolution and more reproducibility from batch-to-batch and column-to-column.

Unmatched Year-to-Year Reproducibility

SymmetryShield™ columns are reversed-phase columns based on Waters’ patented embedded polar group technology that literally “shields” the silica’s residual silanols from highly basic analytes.
Shield Technology

Improved Peak Shape and Unique Selectivity

Symmetry300™ columns are wide-pore, reversed-phase columns built on the Symmetry^® column platform, the standard relied on by the pharmaceutical industry  for sensitive, rugged and robust HPLC analyses.  These columns:
·         Demonstrate superior performance when simultaneously separating hydrophilic and hydrophobic peptides, as well as neutral and highly basic peptides
·         Perform better at high and low mass load conditions

·         Are the best solution for validation compliance.

Symmetry300 C₄ columns have the highest average peak capacity compared to over 20 leading brands of HPLC columns. In the example shown, a difficult protein separation, the Symmetry300 C₄ column provided a better separation with better peak shape compared to a leading competitive 300Å column. 

Protein: Symmetry300 C₄ Versus Competitors 

Waters Cartridge Column

Waters cartridge system requires re-usable endfittings, which you order when purchasing a Waters HPLC cartridge column for the first time.  Connect these cartridge columns to your HPLC instrument using Waters standard connectors.

Cartridge End-Fittings
2.1 x 50 mm, 2.1 x 100 mm, 2.1 x 150 mm, 2.1 x 250 mm use Part Number 700000117
3.0 x 50 mm, 3.0 x 100 mm, 3.0 x 150 mm, 3.0 x 250 mm use Part Number WAT037525
3.9 x 50 mm, 3.9 x 100 mm, 3.9 x 150 mm, 3.9 x 250 mm use Part Number WAT037525
4.6 x 50 mm, 4.6 x 100 mm, 4.6 x 150 mm, 4.6 x 250 mm use Part Number WAT037525
Waters Sentry Guard Column and Universal Guard Holder
Waters offers a line of Sentry™ Guard columns that contain the same high-performance packing materials as all Waters analytical columns. 
Developed for customers needing increased capacity for biological fluid cleanup and/or temperature control, the Sentry Guard column is available in two holder designs:
·         For use as an integrated part of the Waters steel cartridge column with reusable endfitting
·         For use with any HPLC column.
Both are installed without tools.  
Integrated Sendtry Guard Holder to go with cartridge end-fittings (see above).

3.0 x 20 mm, 3.9 x 20 mm use Part Number WAT046905

Universal Guard Holder (Use on any column)
2.1 x 10 mm use Part Number WAT097958
2.1 x 20 mm use Part Number 186000262
3.0 x 20 mm use Part Number WAT046910
3.9 x 20 mm use Part Number WAT046910
4.6 x 20 mm use Part Number WAT046910