Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs! Waters offers a comprehensive range of analytical system solutions, software, and services for scientists. Liquid Chromatography. Mass Spectrometry. Waters is the leading provider of lab equipment, supplies and software for scientists across the world. Easily research and order everything your lab needs!
Rapid and Simultaneous Analysis of Urinary Catecholamines and Metanephrines Using Mixed-Mode SPE and Hydrophilic Interaction Chromatography (HILIC) for Clinical Research
The extraction and analysis of urinary catecholamines and metanephrines using the Oasis mixed-mode weak cation exchange (WCX) plates and an ACQUITY UPLC BEH Amide Column in HILIC mode is detailed. Extraction using the Oasis WCX Plate resulted in low matrix effects and consistent recoveries for all compounds that translated into excellent analytical precision. HILIC separation resulted in reduced matrix effects for NE and EP and improved resolution between EP and NMT, compared to optimized reversed-phase separations. Quantitative results were excellent, with linear responses from 0.5-500.0 ng/mL and excellent accuracy and analytical precision.
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