This technology brief evaluates the performance of previously published methodology by using either the Xevo™ TQD or Xevo TQ-S micro in MRM mode to detect negative ionizing compounds.

The Xevo TQD and Xevo TQ-S micro Mass Spectrometers are designed to provide rapid, reliable, and reproducible data to deliver consistent low levels of quantitation over a wide dynamic range. When coupled with the ACQUITY UPLC I-Class System, the Xevo TQD and the Xevo TQ-S micro show excellent sensitivity when using a preconfigured targeted MRM qualitative screening method for the analysis of negatively ionizing compounds, such as barbiturates, diuretics, and NSAIDs in urine. The use of dedicated chromatography conditions for negative ionizing compounds allowed for the detection of some compounds, at toxicologically relevant concentrations, which would not have been possible using standard screening conditions. The described sample preparation method can also be applied to other biological matrices such as serum or plasma.