Outstanding Protein SEC Separations

Reliable, high resolving, SEC methods are routinely used in the discovery, development, and quality assessment of protein-based biotherapeutics.  Waters XBridge Protein BEH SEC, 200Å and 450Å, 3.5 µm Columns are ideally suited for HPLC-based, SEC separations of proteins from approximately to 10K to 1,500K Daltons

Higher Throughput Using 3.5 µm SEC Particles

Waters HPLC-based, XBridge Protein BEH SEC, 200Å and 450Å Columns containing diol-bonded, 3.5 µm BEH SEC particles are flow and pressure tolerant for increased sample throughput compared to use of silica-based, SEC columns containing  >5 µm particles. 

Outstanding Column Life

The combination of the Waters stable BEH base particle and innovative diol bonding technology results in reproducible performance due to a stable particle and packed column bed that delivers column  lifetime unheard of in traditional size exclusion chromatographic columns.

Unmatched SEC Column Consistency

Stringent manufacturing quality delivers confidence in SEC column generated data.  Each manufactured batch of XBridge Protein BEH SEC, 200Å and 450Å, 3.5 μm material is extensively tested including with relevant proteins to help ensure unmatched batch-to-batch consistency for supreme confidence in data collected from validated methods.

BEH SEC Protein Performance Standards

Waters recommends benchmarking your HPLC-based, SEC system with the BEH200 SEC Protein Standard Mix or BEH450 SEC Protein Standard Mix throughout the lifetime usage of your column. These standards are the same ones used to quality control check all our SEC column batches and allow you to:

• Verify the performance of your column upon receipt
• Monitor the condition of the columns for extended use troubleshoot separation difficulties that may arise

Protein Characterization Methods

In 2010, Waters first introduced ACQUITY UPLC Protein BEH SEC Columns containing 1.7 µm particles designed for optimal performance on Waters low dispersion, UPLC instrumentation. For the first time, these columns delivered outstanding component resolution and in less time compared to use of traditional SEC column containing 5–8 µm particles. Waters XBridge Protein BEH SEC, 200Å and 450Å, 3.5 μm Columns can now effectively deliver comparable component resolution, on HPLC platforms, where high sample throughput is not a driving requirement.