Specific for small molecule work, experiments with scaling of a system from HPLC to UPLC in this application area, this is a 7 compound mix of  Acetominophen, Acetomidophenol, Acetanilde, Acetylsalicylic acid, Caffeine, Phenacetin, and Salicylic Acid.

The System Evaluation Matrix #1 is intended for use as a reproducible background signal in the evaluation of LC and MS system performance. It is especially suitable for standard small molecule applications where a complicated matrix is required but naturally occurring alternatives, such as urine and plasma, are impractical.

Analysis of Metabolites in System Evaluation Matrix #1.

The MetID standard is a 3 component mix of omeprazole and 2 metabolites used for validation of the Waters Metabolite Identification system and software.