BioResolve SCX mAb Consumables

Charge variant profiling is one of several important techniques performed to assess the efficacy and safety of therapeutic monoclonal antibodies. To address this need, Waters developed BioResolve SCX mAb, 3 μm, corrosion–resistant columns which contain non-porous, polymer-based particles grafted with a rigorously-optimized, multi-component network of negatively charged sulfonic acid ligands. This innovative column technology delivers reproducible, high-resolution, charged-based separations of mAbs and other proteins in LC and LC-MS applications using robust salt or pH gradient methods.

• Strong-cation exchanger based on a novel polymeric composition, hydrophilic coating, and multi-component surface chemistry
• Based on a non-porous, 3 μm particle for optimal diffusion kinetics; high pressure tolerance; and amenability to HPLC, UHPLC, and UPLC Systems
• Available VanGuard FIT Technology that maximizes column life without degrading biomolecule component resolution
• Available pH Gradient Buffer Concentrates to assist in establishing a robust and reproducible “platform method”
• Quality control tested with the mAb Charge Variant Standard to help ensure batch-to-batch consistency
• "More Than Just a Column."

BioResolve CX pH Buffer Concentrates

Designed alongside the BioResolve SCX mAb Columns, these buffer concentrates are:

• Patent pending, pH gradient, system mobile phases for simple-to-implement charge variant profiling
• Carefully optimized pH 5.0 to 10.2 gradients for use with the prototype cation-exchange column to achieve high resolution, robust separations
• 100 mL 10x concentrates for the preparation of mobile phase using a simple 10-fold water dilution

IonHance CX-MS pH Buffer Concentrates

Developed for use with the BioResolve SCX mAb, 3 μm Columns, and designed to deliver robust charge-based separations and high-quality, MS-spectral data.

• Specifcally formulated for reproducibile LC-MS analyses of Intact mAb and mAb IdeS digests
• Deliver cleaner spectra, significantly reduce non-desired noise, super charging, and ion suppression
• Easy to use. Simply add high-quality, MS-grade water to the 10x buffer concentrates that are shipped in 1 L MS-certified LDPE bottles to minimze buffer contamination

mAb Charge Variant Standard

This standard is specifically designed to help benchmark charge variant chromatography when using the BioResolve SCX mAb Columns. It contains a unique mix of intact proteins relevant to biopharmaceutical customers: trytophan, conalbumin, and the NIST RM 8671 (NIST mAb).

In addition, check out our other product offerings for IEX test standards that were designed for either cation- or anion-exchange chromatography with a different set of three proteins that are used to test the Protein-Pak Column line.

Auto•Blend Plus Technology

Auto•Blend Plus Technology significantly reduces the time you spend in the prep lab by automating the formulation of mobile phases from reservoirs of pure solvents or concentrated stock solutions. It enables you to take full advantage of the ACQUITY UPLC H-Class System's quaternary blending capabilities. Auto•Blend Plus manages the desired pH and ionic strength requirements for the selected mobile phase.

Auto•Blend Plus is a standard feature of the ACQUITY UPLC H-Class, ACQUITY Arc, and ACQUITY UPLC H-Class Bio Systems.

Protein-Pak Hi Res Ion-Exchange (IEX) Columns

Protein-Pak Hi Res IEX Columns were developed to characterize recombinant proteins, monoclonal antibodies, and other biological compounds. Their non-porous, high compound binding capacity yields outstanding resolution of charged species in less time than traditional porous IEX offerings.

• Designed for the characterization of protein charge variants
• Two cation-exchangers (carboxymethyl and sulfopropyl) and one anion-exchanger (quaternary ammonium) address differing selectivity needs
• Non-porous, high-capacity stationary phases deliver fast separations that address high-throughput needs

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Protein-Pak HR Ion-Exchange Glass Columns

Protein-Pak HR Ion-Exchange Glass Columns are packed with rigid, polymethacrylate particles with large 1000 Å pores. The naturally hydrophilic polymer reduces non-specific adsorption, resulting in quantitative recovery of proteins while maintaining bioactivity. The Protein-Pak HR material is stable at pH values from 2 to 12 and withstands exposure to caustic solutions.

Protein-Pak HR series packing materials include:

• Q: Strong anion-exchanger
• DEAE: Weak anion-exchanger
• SP: Strong cation-exchanger
• CM: Weak cation-exchanger

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BioSuite Ion-Exchange HPLC Columns

Cation and anion-exchange columns (polymethacrylate based)

BioSuite Ion-Exchange (IEX) Columns include four separation chemistries -- SP strong and CM weak cation-exchangers, and Q strong and DEAE weak anion-exchangers -- bonded to a pH stable (pH 2–12), methacrylic ester-based polymeric resin. This broad choice of separation chemistries provides chromatographers with the flexibility to develop methods that separate proteins or peptides based on subtle charge differences. Selected BioSuite IEX Columns are also offered with PEEK hardware as well as in 21.5 mm I.D. for lab-scale preparative applications.

Advanced Purification Glass Columns

Waters Advanced Purification (AP) Glass Columns are constructed of biocompatible glass and polymeric materials for use with silica, polymer, or soft gel packings. 

Each column incorporates a distributor to optimize flow and ensure uniform sample distribution onto the packed bed. A replaceable filter protects the packing from large particulate contaminants. 

Available in a variety of sizes, AP Glass Columns use the same design to ensure predictable methods transfer among them. These columns are compatible with analytical and preparative LC instrumentation.

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