The MassPREP™ Phosphopeptide Enrichment Kit, developed for selective enrichment of phosphopeptides from complex samples, includes a 96-well micro-scale solid-phase extraction (SPE) plate packed with an affinity sorbent. The kit also includes a unique chemical (Enhancer™) that you can add to further improve phosphopeptide selectivity.
In contrast to currently popular immobilized metal-affinity chromatography (IMAC) technology, this isolation method does not require pre-loading the sorbent with metal chelator. This phosphopeptide enrichment protocol is simplified. The sorbent has high native affinity towards phosphopeptides and the Enhancer yields superior selectivity of the sorbent towards phosphopeptides, making this a robust enrichment method.
- Selective enrichment of phosphopeptides from complex proteomic samples
- Highly efficient and reproducible affinity-based enrichment
- Easy to use
- Application in high-throughput analysis
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