Preparative-scale chromatography plays a critical role in applications where compounds must be synthesized, identified, isolated, purified, characterized, screened, and tested. Waters AutoPurification Systems offer scalable prep configurations, whether you want the specificity of mass-directed purification or inclusiveness of a UV system.

The AutoPurification HPLC/MS System provides you with the flexibility of using high-throughput parallel runs for selective mass-directed fraction collection from hundreds of samples. With the AutoPurification HPLC System, run samples around the clock with unprecedented versatility and consistency.

• Take the complexity out of purification with our versatile solutions that are capable of purifying micrograms to multigram quantities.
• Automatically process hundreds of samples - or just a few.
• Robust and reliable systems improve your ability to manage increasing workload demands, including unattended instrument operation.
• Flexible configurations enable easy scale-up from analytical to preparative chromatography.
• FractionLynx Application Manager automates the collection of detected fractions, tracks samples and fractions, and then presents the data in an easy-to-view format. The software can trigger collection using a variety of detector signals including UV/Visible, Evaporative Light Scattering (ELS), MS, and analog.
• User-friendly console and software features help you manage solvents and samples, whether you do manual or automated injections.
• Proprietary Optimum Bed Density (OBD) Column design offers the highest sample loading and unmatched column stability.
• Easy-to-upgrade systems help you bring the latest technologies to your lab.

When fraction collection is based on UV absorbance, multiple fractions may result, requiring further analysis to determine the fraction of interest.

With the AutoPurification HPLC System, run samples around the clock with unprecedented versatility and consistency. The System Fluidics Organizer enables you to easily manage column selection and column regeneration.

The AutoPurification HPLC System includes:

• 2767 Sample Manager
• 2545 Binary Gradient Module
• System Fluidics Organizer
• 2489 UV/Vis Detector
• MassLynx Software with the FractionLynx Application Manager

Whether your company manages preparative LC in a centralized lab or provides purification capabilities in an open access environment, Waters' easy-to-use systems and software allow samples to be run with unattended system operation. This improves productivity by enabling chemists to focus on their results rather than on the instrument.

Waters systems and software make purification easier to manage:

• With Open Access software, chemists can walk up to a system, enter their sample, and have results delivered to their desktop, while an administrator monitors the system and manages instrument access
• Fraction collection software provides full-cycle automation, from evaluation and setting collection thresholds, to purification, to fraction analysis
• Unique method filtering software enables the user to quickly choose a method from the existing library of methods
• Automatically generate gradients without having to manually buffer solvent concentration, and build column cleanup and system flushing into your method
• Solvent monitoring system enables you to use multiple solvent reservoirs, with automated warning responses about instrument status, with intelligent shut-down available
• Remote system status monitor tracks the sample queue, instrument, and solvent and waste container status online

When fraction collection is triggered by mass detection, fewer fractions result since you collect only the peak containing the mass of interest.

The AutoPurification HPLC/MS System provides you with the flexibility of using high-throughput parallel runs for selective mass-directed fraction collection from hundreds of samples.

The AutoPurification HPLC/MS System with the ACQUITY QDa mass detector is designed for everyday mass-directed purification, allowing you to collect more of what you want affordably, and with less work. Full automation and high through-put capabilities are achieved with the system's fluidics layout, and enhanced software features combined with Waters' proprietary Optimum Bed Density (OBD) Column technology for maximum efficiency.

The AutoPurification HPLC/MS System includes:

• 2767 Sample Manager
• 2545 Binary Gradient Module
• System Fluidics Organizer
• 2998 Photodiode Array Detector
• 2424 Evaporative Light Scattering Detector
• ACQUITY QDa Mass Detector or SQ Detector 2
• MassLynx Software with the FractionLynx Application Manager

Managing Fraction Collection

FractionLynx Application Manager provides full-cycle automation for purification, from evaluating and setting collection thresholds, to collection of the detected fractions, to tracking the samples and fractions, to presentation of the data in an easy-to-view format.

The software can trigger collection using a variety of detector signals including UV/Visible, evaporative light scattering (ELS), MS, and analog. Other fraction collection options include one-to-one mapping to retain plate format; secondary collection of peaks that are not of primary concern, but that are not classified as waste; and waste collection.

Sample Evaluation

AutoPurify, a feature of the FractionLynx Application Manager, further automates this process by detecting the presence of the target compound and measures its purity to determine the purification process, from initial analytical screening, through purification, to the analysis of the collected fractions.

AutoPurify automatically selects a focused preparative gradient based on an analytical screening run, which provides better quality purification and eliminates the need for expert manual invention. Automatic determination of the collection threshold on a per-sample basis removes the interference from drifting baselines or high background signals, and increases ease of use. Fractions can be re-injected automatically if required, and information exported.

Identification	Isolation	Confirmation
TIC chromatogram of the analytical-scale analysis of a crude compound indicates partial separation between the compounds.	Successful separation was achieved using a focused gradient; fraction collection is indicated by the shaded area.	TIC chromatogram of the analysis of the collected fraction indicates a successful purification.

Build a preparative LC system to match your needs, workload, and budget. Choose from a variety of components to configure a system that's right for your lab.

Whether your application requires low or high pressure mixing, low or high flow rates, manual or automated injection and collection - you choose the level of functionality and capacity that your application requires. And since Waters Purification Systems are upgradable, you don't have to worry about your investment as your workload increases.

When fraction collection is based on UV absorbance, multiple fractions can result, requiring further analysis to determine the fraction of interest. Run samples around the clock with unprecedented versatility and consistency. Add the System Fluidics Organizer to easily manage column selection and column regeneration.

Multiple detection choices:

• Ultraviolet/visible (UV/Vis) absorbance
• Evaporative light scattering (ELS)
• Photodiode array
• Mass spectrometry (MS)
• Analog

Easily manage sample injection and collection:

• Manual injection
• Automated injection
• Automated fraction collection
• Automated injection and fraction collection

Choose a solvent delivery system that matches your sample's needs:

Make common preparative chromatography calculations with the Preparative OBD Column Calculator.

Waters UPLC® technology has been widely accepted by chromatographers because of its improvements over HPLC in sensitivity, resolution, and speed of separations. Now scientists can use this technology in the sample screening process as a tool to evaluate samples prior to purification as well as a tool for rapid analysis of fractions.

Scaling up from analytical UPLC to preparative HPLC is possible with the use of focused gradients. The efficiency of UPLC can be carried through to purification, offering a substantial increase in throughput and productivity. In a preparative environment, where the compound of interest is being isolated from the other components in the sample, retaining analytical resolution is not as important as isolating and collecting the single compound of interest.

UPLC Analytical Separation	Separation using Focused Gradient
The UPLC separation of a pharmaceutical sample shows the compound of interest eluting at 0.48 min, and is partially resolved from the peak at 0.51 min.	A set of focused gradients is created based on the relationship between percent composition and retention time, using dwell time. The theory is the same for HPLC and UPLC, but the time window for the UPLC gradient is much smaller. Here, separation time was 3 min.