Ostro Pass-Through Sample Preparation Products
A simpler way to cleaner samples
Sample preparation does not need to be time-consuming or complicated with Ostro Pass-Through Sample Preparation Products, which provide a simple solution for phospholipid and protein removal. Requiring minimal to no method development, this technology is quick to implement into your laboratory’s workflow.
The Ostro protein precipitation and phospholipid removal plate produces cleaner sample extracts resulting in more sensitive analyses, higher sample throughput, reduced instrument downtime, and increased laboratory productivity.
Ostro Pass-Through Sample Preparation Products
Specifications
Overview
- Removes proteins, particulates, and more than 95% of phospholipids
- Improves reproducibility for more consistent, robust methods
- Increases throughput with an easy-to-implement protocol
- Works great for biological samples (such as plasma, serum, and whole blood)
- 96-well plate format enables high throughput and is compatible with automation
Recommended Use: For the removal of phospholipids and proteins.
Ostro Pass-Through Sample Preparation Benefits
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Ostro |
PPT |
LLE |
SSLE |
SPE |
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Requires method development |
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Highest analyte selectivity |
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Direct injection of eluates |
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Simple |
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High throughput |
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Phospholipid removal |
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Highest sensitivity |
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Clean extracts |
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Fast |
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Environmentally friendly |
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Ostro has several benefits, especially when compared to Protein Precipitation (PPT), Liquid-liquid Extraction (LLE), Solid-Supported Liquid Extraction (SSLE), and Solid Phase Extraction (SPE). This table illustrates the differences.
Simpler way to implement protein and phospholipid cleanup
Implement protein and phospholipid cleanup easily while delivering high recoveries with the universal single-solvent sample preparation method used in the Ostro Pass-through Sample Preparation Plate. Proven to work with a wide range of acidic, basic, and neutral compounds, this technology provides superior results with improved reproducibility for more consistent, robust methods. Unlike the Protein Precipitation (PPT) method, Ostro Pass-through Sample Preparation Plates remove phospholipids that can build up on the analytical column and foul the mass spectrometry source. Ostro also requires less sample preparation time to obtain optimal recoveries for diverse analyte sets than liquid-liquid extraction (LLE) or solid-supported liquid extraction (SSLE).
Faster sample processing and throughput
Streamline sample processing and optimize sample throughput with Ostro Pass-through Sample Preparation Plates, which require no method development, significantly reduce sample preparation time, are easy to automate, and provide results faster. Ostro’s direct inject capability provides further time savings by eliminating dry-down and reconstitution steps. In contrast to PPT, the Ostro Plate has been shown to effectively remove signal-interfering phospholipids. This provides particular benefits when steep, fast, ballistic gradients are used to analyze large numbers of samples in a short time period. By removing phospholipids, Ostro Sample Preparation Plates prevent them from building up over multiple injections, thus increasing system uptime and overall productivity.
More complete phospholipid cleanup
As a major source of matrix effects, phospholipids contribute to assay variability, decreased sensitivity, and unpredictable mass spectrometry results. Unfortunately, many commonly used sample preparation techniques like PPT and LLE do not remove phospholipids. The Ostro Plate provides the best solution to this dilemma by offering more complete phospholipid cleanup than any other competitive technique and removing variability, even across multiple lots of human plasma – for consistent, comprehensive, superior results.
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