ACQUITY Premier Columns
Stop looking and start seeing – no compromise required
Reduce the risks associated with unpredictable chromatography with ACQUITY Premier Columns with MaxPeak High Performance Surfaces (HPS) Technology, which allow you to see and do more by reducing analyte/surface interactions.
With Waters ACQUITY Premier Columns, chromatographers can harness the separation efficiency improvements and increased flow rates of ultra-performance liquid chromatography (UPLC) technology, improving analyte resolution, sensitivity, business productivity, and profitability. As the next evolution in the ACQUITY UPLC Column family, ACQUITY Premier Columns are built on the industry-leading sub-2-µm particle technologies from Waters you trust.
Looking for faster performance? Check out the ultra-short, high-throughput ACQUITY Premier GlycoProtein Column, and high-throughput ACQUITY Premier Protein SEC Column for faster chromatography.
ACQUITY Premier Columns
Waters offers a wide selection of SEC Column chemistries for HPLC, UHPLC, and UPLC analysis.
View the wide selection of ACQUITY UPLC sub-2-μm Column chemistries.
Specifications
Overview
- Reduces column conditioning and passivation for higher productivity
- Increases reproducibility from analysis to analysis, for more confidence in analytical results
- Reduces non-specific absorption for increased sensitivity, improved peak shape, and easier analyte integration
- Provides superior separation speed, sensitivity, and resolution with best-in-class column efficiency
- 3 UPLC particles of various substrate technologies, including Ethylene Bridged Hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH)
- Reversed-phase and HILIC stationary phases, including C18, Embedded-Polar, and Amide
- Application-specific column chemistries for peptides, proteins, oligonucleotides, and glycan analysis
- Prolonged column lifetimes with VanGuard FIT Columns, and easy-to-use, all-in-one guard/column design
Recommended Use: For increased sensitivity, improved peak shape, and less column equilibration time in methods development.
ACQUITY Premier Column Featured Technologies
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Reduce analyte/surface interactions -
Increase analyte recovery
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Better peak shape
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Increase sensitivity
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All-in-one guard, plus column design
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Achieve longer column lifetimes
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Easy installation
* Specific to columns noted with VanGuard FIT
ACQUITY Premier BEH Column Characteristics
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Universal C18 Column
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Suitable for a diverse range of analytes
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High efficiency
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Industry-leading mobile-phase pH 1–12 and temperature compatibility
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General purpose high efficiency C8 column
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Less hydrophobic than traditional C18 columns
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Suitable for a wide range of analytes
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Superior chemical stability pH 1 – 12
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Highly reproducible; high efficiency phenyl column
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Alternative selectivity to traditional C18 columns
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First choice for polyaromatic compounds
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Superior chemical stability pH 1 – 12
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Contains an embedded-polar group combined with the hydrophobicity of C18
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Complementary selectivity to a C18 column
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Enhanced peak shape for basic compounds
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Compatible with 100% aqueous mobile phases
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High efficiency
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Designed to retain highly polar analytes that are too polar to retain by reversed-phase
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Exceptional chemical stability at high pH and high temperature
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Ideal for sugar/carbohydrate analysis
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Exceptional retention of polar analytes spanning a wide range of polarity
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Strong retention of polar basic analytes
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Superior chemical stability pH 1 -9, when comparted to silica based unbonded HILIC phases
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Highly reproducible and robust HILIC phase
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Orthogonal selectivity to reversed-phase separations
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High efficiency
ACQUITY Premier CSH Column Characteristics
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Universal column choice for UV and MS applications
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Suitable for a broad range of compounds
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Alternate selectivity to BEH C18
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Exceptional peak shape and increased load capacity
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Suited for basic compounds at low pH
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Complementary selectivity to straight-chain-alkyl phases
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Exceptional peak shape for basic compounds under low- and high-pH conditions
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Increased retention for acidic compounds at low pH
ACQUITY Premier HSS Column Characteristics
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Ideal for the enhanced retention of polar and non-polar compounds
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Compatible with 100% aqueous mobile phases
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Mechanically stable at elevated pressures
ACQUITY Premier Application-Specific Column Characteristics
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Sub-2-μm particle columns for UPLC separations
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250 Å pore size
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High throughput & resolution SEC separations of biomolecules ranging from 10,000 to 650,000 Daltons on low dispersion UPLC Systems
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Available SEC Guard Columns to extend analytical SEC Column life
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Significantly reduce non-desired ionic and hydrophobic secondary interaction for desired SEC platform methods with MaxPeak Premier Technology and BEH-PEO SEC particle technology
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Separates proteins of various sizes, hydrophobicities, and isoelectric points
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Tolerates extreme pH and temperature, and provides minimal secondary interactions
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Improves sensitivity for phosphorylated proteins and low-level intact and subunit mAb analysis
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High efficiency separations with 1.7 µm particles
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Suited for the HILIC analysis of neutral and acidic glycans
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Suited for the reversed-phase analysis of hydrophobic and hydrophilic peptides at low and high pH
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Outstanding peak capacity and peak shape in TFA or FA when compared to 100% silica based C18 columns
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Two pore sizes (130Å and 300Å) for a wide range of peptides
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Superior peak shape and reduced tailing for acidic peptides
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Enhanced recovery and sensitivity for acidic peptides
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Outstanding peak capacities with formic acid for MS-based applications
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Excellent performance with TFA for optical based applications
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Less dependence on TFA or FA to achieve optimal peak capacity
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Accepts greater peptide mass loads than many competitive technologies for detection of low-level impurities
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At low pH with the positive charge on CSH, you get less secondary interactions with the particle
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Superior peak shape and reduced tailing for acidic peptides
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Enhanced recovery and sensitivity for acidic peptides
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Separations that require silica-based selectivity
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Separations that necessitate increased peptide retention
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Ideally suited for small, hydrophilic peptides
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Superior peak shape and reduced tailing for acidic peptides
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Enhanced recovery and sensitivity for acidic peptides
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Suited for the analysis of detritylated oligonucleotides using ion-pair, reversed-phase chromatography
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Widepore BEH 300Å C18 columns facilitate long-mer oligo separations for efficient poly A tail analysis and CRISPR sgRNA characterization
Get superior peak shape for phosphorylated and carboxylated analytes
Phosphorylated and carboxylated analytes are metal sensitive and can readily adsorb to stainless-steel surfaces, leading to poor peak shape, low recovery, and a reduction in sensitivity. ACQUITY Premier Columns improved peak shape for these analytes, making it easier for scientists to accurately integrate and quantify these troubling compounds.
Achieve flexibility in method development
Chromatographic laboratories need to quickly and easily develop robust methods, but those methods need to be compatible with all modern chromatographic detection modes and transfer to laboratories and sites on different LC system platforms. With ACQUITY Premier Columns, method development scientists in all application areas can achieve a wide range of selectivities while ensuring high performance, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.
Get superior peak shape for phosphorylated and carboxylated analytes
Phosphorylated and carboxylated analytes are metal sensitive and can readily adsorb to stainless steel surfaces, leading to poor peak shape, low recovery, and a reduction in sensitivity. ACQUITY Premier Columns improved peak shape for these analytes, making it easier for scientists to accurately integrate and quantify these troubling compounds.
Ruggedness you want, the repeatability you need
ACQUITY Premier Oligonucleotide Columns provide superior analyte peak shape, efficiency, and chemical stability for your oligo separations. ACQUITY Premier Oligonucleotide Columns are packed with BEH particle technology and able to operate at an elevated pH – ideal for oligo separations. The MaxPeak HPS Technology in ACQUITY Premier Oligonucleotide Columns improves the peak shape for oligos, making it easier to identify and quantify these analytes.
Achieve flexibility in method development
Chromatographic laboratories need to quickly and easily develop robust methods, but those methods need to be compatible with all modern chromatographic detection modes and transfer to laboratories and sites on different LC system platforms. With ACQUITY Premier Columns, method development scientists in all application areas can achieve a wide range of selectivities while ensuring high performance, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.
Reproducibly generate the highest quality reversed-phase peptide separations
ACQUITY Premier Peptide Columns are designed to increase analyte recovery, sensitivity, and reproducibility by minimizing analyte/surface interactions that can lead to sample loss. Each batch of peptide particle material is chromatographically tested with digested peptides as part of the peptide mapping qualification.
Address the complexity of SEC separations
Experience the advancements in SEC-based technologies to address non-desired specific ionic and hydrophobic interactions for more reliable results with ACQUITY Premier Protein SEC and XBridge Premier Protein SEC Columns and Guards, which are part of the MaxPeak Premier SEC Column family.
With MaxPeak Premier SEC Columns, you can:
Reproducibly resolve injected sample components that are less than 2x different in size for challenging applications
Use a ‘platform SEC method’ to reduce method development time and cost
Ensure batch-to-batch and column-to-column performance
Representative performance of glycan BEH Amide chemistry from the first injection
ACQUITY Premier Glycan BEH Amide Columns with MaxPeak High Performance Surfaces (HPS) Technology reduce sample loss caused by non-specific adsorption on metal surfaces and deliver the representative performance of Glycan BEH Amide chemistry from the first injection. ACQUITY Premier Glycan BEH Amide Columns require no conditioning or passivation, saving time and reagents, and improve recovery of the phosphorylated glycans.
Supreme resolution of charged glycan species
Analyzing acidic glycans is often challenging as they are frequently lost to metallic column surfaces. The ACQUITY Premier Glycan BEH C18 AX Column hardware is outfitted with specialized inert surfaces, MaxPeak High Performance Surfaces. This introduces a barrier layer to prevent acidic glycans from undergoing undesired secondary interactions inside the column. In turn, glycan recoveries are improved, and requirements for sample conditioning upon first time use is eliminated.
Providing an orthogonal technology to HILIC, the ACQUITY Premier Glycan BEH C18 AX Columns are anion-exchange reversed-phase (RP) columns that provide charge-based separations and extra resolution for acidic glycans. With the mixed-mode separation, glycans are grouped by different charged states, and retention is increased to analyte net charge. Additional resolution of isomeric glycan species within the same charged group can be achieved due to the RP properties of the stationary phase.
Applications
Reversed-phase bonded phases typically have poor peak shape for basic compounds when using formic acid, even at analytical mass loads. However, ACQUITY UPLC CSH Columns are the exception. These rugged UPLC Columns provide superior peak shape for basic analytes with formic acid or other low ionic-strength, acidic mobile phases. The controlled, low-level, positive surface charge bonded to the ethylene-bridged hybrid (BEH) particles provides excellent peak shape for bases—without the need for the use of ion-pairing reagents.
ACQUITY UPLC BEH 1.7 μm fully porous columns provide superior analyte peak shape, efficiency, and chemical stability to any separation. ACQUITY UPLC BEH Columns are available in both reversed phase and HILIC separation modes, with chemistries that provide selectivities for a wide range of compounds. With the ruggedness to operate at extreme pH conditions, ACQUITY UPLC BEH Columns enable separation scientists to use a wide pH range to influence retention, selectivity, and sensitivity for ionizable compounds.
Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.
Representative performance of glycan BEH Amide chemistry from the first injection
ACQUITY Premier Glycan BEH Amide Columns with MaxPeak High Performance Surfaces (HPS) Technology reduce sample loss caused by non-specific adsorption on metal surfaces and deliver the representative performance of Glycan BEH Amide chemistry from the first injection. ACQUITY Premier Glycan BEH Amide Columns require no conditioning or passivation, saving time and reagents, and improve recovery of the phosphorylated glycans.
Supreme resolution of charged glycan species
Analyzing acidic glycans is often challenging as they are frequently lost to metallic column surfaces. The ACQUITY Premier Glycan BEH C18 AX Column hardware is outfitted with specialized inert surfaces, MaxPeak High Performance Surfaces. This introduces a barrier layer to prevent acidic glycans from undergoing undesired secondary interactions inside the column. In turn, glycan recoveries are improved, and requirements for sample conditioning upon first time use is eliminated.
Providing an orthogonal technology to HILIC, the ACQUITY Premier Glycan BEH C18 AX Columns are anion-exchange reversed-phase (RP) columns that provide charge-based separations and extra resolution for acidic glycans. With the mixed-mode separation, glycans are grouped by different charged states, and retention is increased to analyte net charge. Additional resolution of isomeric glycan species within the same charged group can be achieved due to the RP properties of the stationary phase.
Reproducibly generate the highest quality reversed-phase peptide separations
ACQUITY Premier Peptide Columns are designed to increase analyte recovery, sensitivity, and reproducibility by minimizing analyte/surface interactions that can lead to sample loss. Each batch of peptide particle material is chromatographically tested with digested peptides as part of the peptide mapping qualification.
Address the complexity of SEC separations
Experience the advancements in SEC-based technologies to address non-desired specific ionic and hydrophobic interactions for more reliable results with ACQUITY Premier Protein SEC and XBridge Premier Protein SEC Columns and Guards, which are part of the MaxPeak Premier SEC Column family.
With MaxPeak Premier SEC Columns, you can:
Reproducibly resolve injected sample components that are less than 2x different in size for challenging applications
Use a ‘platform SEC method’ to reduce method development time and cost
Ensure batch-to-batch and column-to-column performance
Ruggedness you want, the repeatability you need
ACQUITY Premier Oligonucleotide Columns provide superior analyte peak shape, efficiency, and chemical stability for your oligo separations. ACQUITY Premier Oligonucleotide Columns are packed with BEH particle technology and able to operate at an elevated pH – ideal for oligo separations. The MaxPeak HPS Technology in ACQUITY Premier Oligonucleotide Columns improves the peak shape for oligos, making it easier to identify and quantify these analytes.
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