MaxPeak Premier Protein SEC Columns
More confident biological compound separations start here.
Get superior size-based separations of biological compounds with MaxPeak Premier Protein SEC Columns.
Size-exclusion chromatography (SEC) is a powerful technique for aggregate and fragment analysis in the research, development, and manufacturing of biotherapeutic proteins, such as insulin and monoclonal antibodies (mAb).
Experience unmatched batch-to-batch reproducibility with the wide range of Waters SEC columns and standards for peptides and proteins characterization.
MaxPeak Premier Protein SEC Columns
Waters offers a wide selection of SEC Column chemistries for HPLC, UHPLC, and UPLC analysis.
Waters offers a wide variety of size-based separations for peptides, proteins, and viral vectors.
Specifications
Overview
- Get 30% improved resolution with Waters MaxPeak Premier Protein SEC Columns
- Eliminate the need for extensive method development with a one-size-fits-all method in your lab
- Get unmatched batch-to-batch reproducibility with the wide range of Waters SEC columns and standards for peptide and protein characterization
- Limit unwanted secondary interactions that lead to broader peaks, inaccurate quantitation of aggregates, and increased tailing
Recommended Use: Platform method for protein size variant analysis from 10-650 KDa
Features Header
30% improved resolution
- MaxPeak Premier Columns deliver highly efficient separations that enable you to resolve species that vary by less than 2X in size on any LC
- Achieve narrow peak widths with reduced tailing providing accurate quantitation of aggregates and fragments of <0.5% total peak areas and 30% better resolution
- Gain efficiency for proteins ranging from 10 KDa to 650 KDa, leveraging MaxPeak Premier Technology coupled with BEH-PEO Particle Technology
More confident platform methods
- Eliminate the need for extensive method development by using a one-size- fits-all method in your lab
- Ensure accurate results, such as high molecular weight (HMW) percentage, which should not depend on mobile phase choice
- Resolve protein size variants for over 80% of proteins without the need for additives with a Phosphate Buffered Saline (PBS) eluent
Exceedingly inert for biologics
SEC should separate proteins based exclusively on size, however unwanted secondary interactions can lead to broader peaks, inaccurate quantitation of aggregates, and increased tailing. Waters has industry-leading innovations to limit secondary interactions on both column and particles:
- MaxPeak High Performance Surface columns mitigates ionic interactions between hardware and proteins
- Ethylene Bridged Hybrid (BEH) SEC particles with high coverage hydroxy-terminated polyethylene oxide (PEO) bonding minimizes hydrophobic particle interactions
- MaxPeak High Performance Surfaces enable out-of-the-box performance reducing the need for lengthy column conditioning typically observed in other columns
Stay compliant with smart column options
Ideal for compliant laboratories, smart column options are available for identifying and tracking column information. The eCord is a data recording device which is permanently attached to the column body of 2.x µm particle size (2.1 and 3.0 mm internal diameter) and ACQUITY UPLC columns and is standard for those column offerings. Rather, the eConnect column tag is a permanently attached electronic tag device for HPLC columns (≥2.x µm particles) used with Alliance iS and Alliance iS Bio HPLC Systems that are optional. Data from column devices (eCord device or eConnect column tag) can be accessed using the respective system’s console. These smart-column abilities provide a quick overview of column history and can be utilized as a basic troubleshooting tool. Data residing on these devices are automatically imported by and later searchable via Empower CDS.
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