ACQUITY Premier System with Multi-Dimensional Technology

Number of solvents

Up to four, in any combination of two: Al or A2 and B1 or B2

Solvent conditioning

Five vacuum degasser, one allocated for injector purge solvent

Operating flow rate range

0.001 to 2.000 mL/min, in 0.001 mL increments (firmware v1.71 and later)

Compressibility compensation

Automatic and continuous, no user intervention required

Plunger seal wash

Integral, active, programmable

Gradient profiles

11 gradient curves [including linear, step (2), concave (4), and convex(4)]

Wet prime

Automatic

Maximum operating pressure

15,000 psi up to 1 mL/min, 9000 psi up to 2 mL/min per pump, not more than 15,000 psi total

Composition accuracy

±0.5% absolute (full scale) from 5% to 95% of flow rates from 0.50 to 2.00 mL/min

Composition precision

0.15% RSD or ±0.01 min SD, whichever is greater, based on retention time

Flow precision

0.075% RSD or ±0.02 min SD, or 1.00 s for run times less than 1.00 min based on retention time or volumetric measures (0.5 to 2.0 mL/min)

Primary wetted materials

Titanium, PPS, fluoropolymer, fluoroelastomer, UHMWPE blend, sapphire, ruby, zirconia, Nitronic 60, DLC, PEEK and PEEK blend, Inconel 600, FEP

Solvent capacity

Blend up to four solvents in any combination (standard); total capacity of nine solvents with integrated solvent select valve (optional)

Number of fluidic paths

Three (Path 1, Path 2, and waste), with Arc™ Multi-flow path technology (standard)

Dwell volume selection

Automated with Arc Multi-flow path technology

Solvent conditioning

Integrated vacuum degassing, four chambers

Gradient formation

Low-pressure mixing, quaternary gradient

Gradient profiles

11 gradient curves [including linear, step (2), concave (4), and convex (4)]

Check valves

Passive check valves

Flow accuracy

+/- 1.0% at 0.5, 3.0, and 5.0 mL/min

Flow precision

≤0.075% RSD or +/-0.020 min SD, whichever is greater, based on six replicates [60:40 water:methanol pre-mixed; 1.5 mL/min; alkylphenone mix; 24.0 μL injection volume; CORTECS C₁₈ 2.7 μm, 4.6 x 50 mm; 35 °C; UV @254 nm]

Composition ripple

≤0.5 mAU [mobile phase containing 0.1% TFA in water/acetonitrile; 1.5 mL/min; CORTECS C₁₈ 2.7 μm, 4.6 x 50 mm; 35 °C; UV @214 nm]

Composition accuracy

+/- 0.5% absolute (full scale) from 5 to 95%; 0.5 to 5.0 mL/min [methanol; methanol with 5.0 mg/mL caffeine step gradient; UV @273 nm]

Composition precision

+/- 0.15% RSD or 0.04 min SD, whichever is greater based on six replicate injections [60:40 water:methanol via Auto•Blend™ Technology; 0.5 mL/min; alkylphenone mix; 24.0 μL injection volume; CORTECS C₁₈ 2.7 μm, 4.6 x 50 mm; 35 °C; UV @254 nm]

Compressibility compensation

Automatic and continuous

Priming

Wet priming can run at flow rates up to 10 mL/min

Pump seal wash

Standard

Primary wetted materials

316L stainless-steel, PPS, fluoropolymer, UHMWPE blend, sapphire, ruby, zirconia, DLC, PEEK and PEEK blend, titanium alloy

Injection volume range

0.1 to 10.0 μL as standard

Up to 1000.0 μL with optional extension loops

Accuracy (aspiration)

±0.2 μL (measured by fluid weight removed from vial with 10 μL injections averaged over 20 injections using standard 100 μL syringe)

Linearity†

≥999 (standard needle)

Precision†

≤0.25% RSD, 5 to 100 μL

Number of sample plates

Any two of the following:

96 and 384 microtiter plates

48-position 2.00 mL vial plates

48-position 0.65 mL micro-centrifuge tube plates

24-position 1.50 mL micro-centrifuge tube plates

Maximum sample capacity

768 in two 384-well plates or 96 in 2 mL vial holders, four additional positions for dilution functions

Sample compartment temp.

4.0 to 40.0 °C, settable in 0.1 °C increment

Temperature accuracy

±0.5 °C at sensor

Temperature stability

±1.0 °C at sensor

Sample manager heat time

≤30 min ambient -40 °C

Injection needle wash

Integral, active, programmable

Minimum sample required

3 μL residual, using Waters Total Recovery 2 mL vials (zero offset)

Sample carryover†

≤0.002% caffeine (UV)

≤0.002% sulphadimethoxine (MS)

Advanced sample

manager capabilities

Auto-dilution, auto-addition, and load-ahead

Primary wetted materials

Vespel SCP, PEEK blend, DLC, and HPS