XBridge Premier Columns
Particle Stability with MaxPeak HPS Protection
Working at the extremes of temperature and pH while maximizing analyte peak shape is a significant challenge for methods development scientists. Now, with XBridge Premier 2.5 µm and 3.5 µm Columns, it doesn’t have to be.
XBridge Premier Columns prevent undesired analyte/surface interactions through the power of MaxPeak HPS Technology, which is designed to increase analyte recovery, sensitivity, and reproducibility by minimizing analyte/surface interactions that can lead to sample losses.
XBridge Premier Columns are the evolution of Waters XBridge XP (eXtended Performance) columns and XBridge HPLC columns, and they combine the industry-leading column stability of Ethylene Bridged Hybrid (BEH) particles with MaxPeak High Performance Surfaces (HPS) Technology.
XBridge Premier Columns
XBridge Columns are available in a broad range of sorbent particles and sizes for HPLC, UHPLC.
Specifications
Overview
- Improves analyte sensitivity and peak shapes for more confidence in analytical results
- Reduces column conditioning and passivation for higher productivity
- Provides high pH stability and chemical resistance for reliability and longevity
- Enables maximum method development flexibility
- Prolongs column lifetimes with optional VanGuard FIT format
Recommended Use: For method development flexibility with improved analyte peak shape and recovery.
XBridge Premier Column Featured Technologies
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Reduce analyte/surface interactions -
Increase analyte recovery
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Better peak shape
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Increase sensitivity
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Rugged hybrid organic/inorganic base particle
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Improved peak shape for bases- low silanol activity
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Wider operating pH range for method development flexibly
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Mechanical strength for maximum column efficiency at UHPLC operating pressures
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All-in-one guard, plus column design
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Achieve longer column lifetimes
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Easy installation
* Specific to columns noted with VanGuard FIT
XBridge Premier BEH Column Characteristics
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General purpose reversed-phase C18 Column
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Superior chemical stability pH 1–12
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Elevated operating temperature, up to 90 °C
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Reduction in analyte/surface interactions
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Excellent pH stability
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VanGuard FIT Column option
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Alternative selectivity to traditional alkyl chain C18 columns
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Improved basic analyte peak shape near neutral pH
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Compatible with 100% aqueous mobile phases
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Reduction in analyte/surface interactions
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VanGuard FIT Column option
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General purpose HILIC column
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Ideal chemistry for the separation of carbohydrates/saccharides
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Excellent chemical stability pH 2–11
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Elevated operating temperature, up to 90 °C
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Reduction in analyte/surface interactions
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VanGuard FIT Column option
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General purpose C8 column
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Less hydrophobic than traditional C18 columns
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Suitable for a wide range of analytes
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Superior chemical stability pH 1–12
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Highly reproducible phenyl column
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Alternative selectivity to traditional C18 columns
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First choice for polyaromatic compounds
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Superior chemical stability pH 1–12
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Strong retention of polar basic analytes
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Superior chemical stability pH 1 -9, when comparted to silica based unbonded HILIC phases
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Highly reproducible and robust HILIC phase
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Orthogonal selectivity to reversed-phase separations
XBridge Premier BEH-PEO Column Characteristics
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2.5 μm particle columns for HPLC and UHPLC separations
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250 Å pore size
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High resolution SEC separations of biomolecules ranging from 10,000 to 650,000 Daltons on any LC system
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Available SEC Guard Columns to extend analytical SEC Column life
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Significantly reduce non-desired ionic and hydrophobic secondary interaction for desired SEC platform methods with MaxPeak Premier Technology and BEH-PEO SEC particle technology
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Specifically designed and QC tested with NIST-based, mAb Size Variant Standard to help ensure batch-to-batch consistency
XBridge Premier HPLC and UHPLC Application-Specific Column Characteristics
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Suited for the reversed-phase analysis of hydrophobic and hydrophilic peptides at low and high pH
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Outstanding peak capacity and peak shape in TFA or FA when compared to 100% silica based C18 columns
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Two pore sizes (130 Å and 300 Å) for a wide range of peptides
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Superior peak shape and reduced tailing for acidic peptides
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Enhanced recovery and sensitivity for acidic peptides
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Separates proteins of various sizes, hydrophobicities, and isoelectric points
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Tolerates extreme pH and temperature, and provides minimal secondary interactions
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Improves sensitivity for phosphorylated proteins and low-level intact and subunit mAb analysis
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2.5 µm particles for use on UPLC, UHPLC, and HPLC separations
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Suited for analyzing released and 2-aminobenzamide (2-AB) labeled N-linked glycans from various biotherapeutics
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Suited for the HILIC analysis of neutral and acidic glycans
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Suited for the analysis of detritylated synthetic oligonucleotides using ion-pair, reversed-phase chromatography
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Widepore BEH 300Å C18 columns facilitate long-mer oligo separations for efficient poly A tail analysis and CRISPR sgRNA characterization
Mechanical strength and chemical stability
Ethylene Bridged Hybrid (BEH) particle technology ensures maximum column performance and longer column lifetimes under all chromatographic conditions. The hybrid particle technology offers many advantages over conventional silica-based particles, including the ability to control silanol activity for better reproducibility, peak shape, and efficiency. The BEH particle is created from two high purity monomers: tetraethoxysilane (TEOS) and bis(triethoxysilyl) ethane (BTEE), which results in highly stable, mechanically strong particles that set the performance standard for method development.
Cost-effective column protection
VanGuard FIT (Fully Integrated Technology) Guard Columns provide an easy-to-use format that extends the life of your column without sacrificing performance. They thread directly into a FIT column that has been precisely machined to integrate them into the column flow path, eliminating excess volumes. This easy-to-install design does not require ferrules or other small parts.
*XBridge Premier VanGuard FIT Columns are required for use with the XBridge Premier VanGuard FIT Guard Columns, as this is an integrated design requiring the two to thread together.
Stay compliant with smart column options
Ideal for compliant laboratories, smart column options are available for identifying and tracking column information. The eCord is a data recording device which is permanently attached to the column body of 2.x µm particle size (2.1 and 3.0 mm internal diameter) and ACQUITY UPLC columns and is standard for those column offerings. Rather, the eConnect column tag is a permanently attached electronic tag device for HPLC columns (≥ 2.x µm particles) used with Alliance iS and Alliance iS Bio HPLC Systems that are optional. Data from column devices (eCord device or eConnect column tag) can be accessed using the respective system’s console. These smart-column abilities provide a quick overview of column history and can be utilized as a basic troubleshooting tool. Data residing on these devices are automatically imported by and later searchable via Empower CDS.
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