GlycoWorks RapiFluor-MS Released N-Glycan Kits
Rapid, sensitive and easy detection of N-glycans
Reimagine the workflow for released N-glycan analysis with GlycoWorks RapiFluor-MS N-glycan Kits. With the technology behind these kits, you can achieve unprecedented fluorescent and mass spectrometric performance for glycan detection – all while improving the throughput of N-glycan sample preparation.
With GlycoWorks RapiFluor-MS N-glycan Kits, you don’t have to compromise between speed and sensitivity in sample preparation—and you’ll gain confidence in your analytical results.
GlycoWorks Kits
Specifications
Overview
- DMF-free GlycoWorks RapiFluor-MS Eco Labeling Kits now available! DMSO, a greener alternative to DMF, is substituted for DMF in the standard kits. (Both versions will continue to be available.)
- Achieve unprecedented sensitivity for fluorescence and mass spectrometry
- Save time with a simplified and reproducible 30-minute, 3-step workflow
- Maximize productivity with automation across multiple platforms
- Gain valuable information from characterization to routine monitoring with one label
- Reduce complicated, time-consuming sample preparations
- Support legacy 2-AB methods with dedicated kits and provide easy transfer to RFMS and training for analysts
- Complete your workflow with Waters Glycan Columns and Standards & Buffers
Recommended Use: For fast, efficient, and accurate N-glycan analysis.
Features Header
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Deglycosylation Module: Rapid PNGase F, customized buffer, RapiGest SF Surfactant, and the Intact mAb Mass Check Standard as a control to benchmark the protocol -
Labeling Module: RapiFluor-MS Reagent and Reagent Solvent (DMF or DMSO)
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Clean-up Module: GlycoWorks HILIC µElution Plate* and the GlycoWorks Elution Buffer and Sample Diluent
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Sample Collection Module: Tubes, caps, collection tray, and waste tray needed to handle samples
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Sample cleanup devices with HILIC (amino-propyle) sorbent
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High-throughput: GlycoWorks HILIC µElution SPE Plate* (96-well)
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Single Use: GlycoWorks HILIC 1 cc (20 pack) and GlycoWorks HILIC 1 cc Flangeless Cartridges for the positive pressure manifold (20 pack)
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GlycoWorks Reagent Kit with material for protein deglycosylation and labeling
* A vacuum manifold or positive pressure processor is required for the µElution Plate.
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In response to new regulations restricting the use of dimethylformamide (DMF) , DMF-free GlycoWorks RapiFluor-MS N-glycan Labeling Kits are available with dimethylsulfoxide (DMSO) as the labeling solvent
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Achieve the same unprecedented sensitivity for fluorescence and mass spectrometry as with the conventional DMF-containing kits
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Same 30-minute, 3-step workflow when combined with the deglycosylation and Clean-Up Kits
Save time with a simplified 30-minute, 3-step workflow
Increased fluorescence and MS signal with decreased sample prep time
RapiFluor-MS Label provides increased fluorescence quantification along with unprecedented mass spectral signal intensity. Easily assign and characterize low abundance glycans by serial fluorescence and mass spectrometric detection, using less sample while still obtaining increased sensitivity.
When labeled glycans were analyzed by HILIC chromatography, RapiFluor-MS Label produced the following results:
- Commercial rapid tagging analog of aminobenzamide (AB); 2x greater fluorescence signal and 1000x higher MS signal
- Conventional reductive amination with 2-AB; 4x greater fluorescence signal and 160x higher MS signal
Increased robustness for continuous results
The GlycoWorks RapiFluor-MS N-Glycan Kit enables analysts to perform a high yielding sample preparation with quantitative recovery that ensures accurate and repeatable profiling of N-glycans that is highly comparable to HPLC, 2-AB based methodologies.
Click here to see a demonstration of the robustness of RapiFluor-MS N-glycan sample preparation used together with Glycan BEH Amide HILIC Column chromatography.
Improved recoveries for disulfide rich complex molecules
N-glycans are routinely monitored for the quality of biotherapeutics because they affect the safety and efficacy of many therapeutic proteins. Current glycan analysis is developed with the well-studied glycans from monoclonal antibodies (mAb). There is a complementary method that provides a reproducible profile of N-glycans released from Human Chorionic Gonadotrophin (hCG), a highly complex glycoprotein with multiple disulfide bonds.
Click here to see how the addition of DTT, a reducing reagent, can be added for complete protein denaturation, without extending protocol length.
Increased throughput for N-glycan analysis
RapiFluor-MS dramatically reduces overall sample preparation times and greatly improved MS response when coupled with the ACQUITY UPLC H-Class Bio System and the ACQUITY QDa Detector provides an approach for accurately monitoring released N-glycan species.
Click here to see how you can rapidly monitor Released-N-Glycan profiles during process development using GlycoWorks RapiFluor-MS.
Improved recovery with phosphoglycan SPE buffer
The loss of labeled acidic glycans – especially phosphorylated glycan species – during solid-phase extraction (SPE) purification is a challenge to accurately monitoring biotherapeutic glycosylation. The GlycoWorks Phosphoglycan SPE Elution Buffer, optimized with citrate additive, facilitates the elution, and recovery of phosphorylated glycans to achieve maximum yield.
Offerings for reductive amination (2-AB)
The GlycoWorks Sample Preparation consumable line along with the ACQUITY UPLC BEH Glycan Column is optimized for use with ACQUITY UPLC Systems with fluorescence detection (FLR) for separating the released glycans of biopharmaceuticals as their 2-aminobenzamide (2-AB) derivatives.
The GlycoWorks Sample Preparation consumable line is specifically for 2-AB labeled N-glycans. This consumable line works especially well for the market of biopharmaceuticals, including Immunoglobulin G (IgG). The GlycoWorks Sample Preparation consumable line is not limited to IgG, but it has been validated with IgG.
Deliver optimal results with flexibility and automation
Waters offers a variety of solutions for workflow automation including the Andrew+ Pipetting Robot and OneLab Software. Additionally, verified starter scripts for the GlycoWorks RapiFluor-MS method are available on Tecan and Hamilton platforms.
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