XSelect Columns
Achieve unmatched reproducibility with a wide range of selectivity
Quickly and easily develop robust methods compatible with all modern chromatographic detection modes and transfer them from lab to lab with the XSelect Column family.
Designed to help you achieve reproducible and highly selective chromatographic separations, the XSelect Column family features two base particles technologies, Charged Surface Hybrid (CSH) and High Strength Silica (HSS). With XSelect Columns, method development scientists like you can achieve a wide range of selectivities across all application areas, without compromising performance.
XSelect Columns
Unwanted interactions between analytes and metal ions?
XSelect Premier Columns with MaxPeak HPS technology mitigate interactions with stainless-steel column hardware.
Specifications
Overview
- Designed for selectivity and stability at low pH
- Superior peak shape for basic compounds
- Highest analyte mass loading available
- Excellent batch-to-batch reproducibility
- Ideal for rapid method development
- CSH Column chemistries: C18, Fluoro-Phenyl (PFP), and Phenyl-Hexyl, available in 2.5 XP, 3.5, 5 μm analytical and 10 μm OBD preparative sizes
- HSS Column chemistries: HSS C18, C18 SB, and T3, available in 2.5 XP, 3.5, and 5 μm particle sizes
- Prolonged column lifetimes with optional VanGuard Cartridges specifically designed using XSelect stationary phases
Recommended Use: For improving selectivity, peak shape, and retentivity in methods development.
XSelect CSH Column Characteristics
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Alternative selectivity to existing C18 columns -
Low anion exchange
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High efficiency
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Excellent pH stability
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Ideal for improved peak shape for bases with low pH mobile phase
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General purpose hybrid column
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Alternative selectivity for aromatic compounds
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Medium anion exchange
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High efficiency
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Excellent pH stability
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Ideal for improved peak shape for bases with low pH mobile phase
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General purpose hybrid column
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Alternative selectivity for acids and bases
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High anion exchange
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High efficiency
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Excellent pH stability
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Ideal for improved peak shape for bases with low pH mobile phase
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General purpose hybrid column
XSelect HSS Column Characteristics
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High efficiency for acids, bases, neutrals
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Excellent low pH stability
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General purpose silica columns
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High retention
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Excellent retention for polar analytes
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100% water wettable
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High retention
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High efficiency
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Excellent low pH stability
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Alternative selectivity for bases
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Increased cation exchange at mid pH
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General purpose silica columns
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High retention
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High efficiency
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Excellent low pH stability
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Alternative selectivity for polar bases
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Alternative selectivity for aromatic compounds
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General purpose silica columns
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High retention
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High efficiency
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Excellent low pH stability
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Stable and reproducible cyano chemistry
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Alternative selectivity and increased retention for polar compounds
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General purpose silica columns
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High retention
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High efficiency
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Excellent low pH stability
XSelect HPLC and UHPLC Application-Specific Column Characteristics
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Outstanding peak capacities with formic acid for MS-based applications
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Excellent performance with TFA for optical based applications
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Less dependence on TFA or FA to achieve optimal peak capacity
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Accepts greater peptide mass loads than many competitive technologies for detection of low-level impurities
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At low pH with the positive charge on CSH, you get less secondary interactions with the particle
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Separations that require silica-based selectivity
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Separations that necessitate increased peptide retention
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Ideally suited for small, hydrophilic peptides
Increase selectivity across compounds
Using selectivity and retentivity to influence chromatographic behavior is common in method development. With the choice of phases in the XSelect HPLC and UHPLC family, you can achieve selectivity and retention across a wide variety of compounds by using different base particles.
The XSelect HSS and CSH particles provide enhanced selectivity for acidic, alkaline and neutral compounds. The XSelect CSH Phenyl-Hexyl Column places more emphasis on reversed-phase interactions compared to secondary Lewis-base interaction. The XSelect HSS C18 SB Column was engineered to provide enhanced retention for basic polar compounds using low-pH mobile phases. When compared to a standard C18 phase, XSelect HSS T3 Columns provide increased retention for polar compounds.
Powerful tools for your method development
The scalability and versatility of XSelect CSH Columns enables you, the isolation and purification scientist, to use them as powerful method development tools. The hybrid materials used to create the CSH stationary phases are known industry-wide for being the most pressure-tolerant columns on the market. Rugged performance combined with Optimum Bed Density (OBD) packing technology produces columns that have exceptional column lifetimes under a wide range of operating conditions. With the columns’ scalable design, you can choose from larger to smaller and narrower preparative columns to reduce solvent consumption and fraction volumes.
Achieve flexibility in method development
Chromatographic laboratories need to quickly and easily develop robust methods that are compatible with all modern chromatographic detection modes and transferrable to laboratories and sites that operate different LC system platforms. With ACQUITY UPLC CSH and XSelect Columns, method development scientists in all application areas can achieve a wide range of selectivities without compromising performance across attributes, including superior peak shape for basic compounds, low column bleeds, excellent batch-to-batch reproducibility, and high efficiency.
Stay compliant with smart column options
Ideal for compliant laboratories, smart column options are available for identifying and tracking column information. The eCord is a data recording device which is permanently attached to the column body of 2.x µm particle size (2.1 and 3.0 mm internal diameter) and ACQUITY UPLC columns and is standard for those column offerings. Rather, the eConnect column tag is a permanently attached electronic tag device for HPLC columns (3 µm particles) used with Alliance iS HPLC System that is optional. Data from column devices (eCord device or eConnect column tag) can be accessed using the respective system’s console. These smart-column abilities provide a quick overview of column history and can be utilized as a basic troubleshooting tool. Data residing on these devices are automatically imported by and later searchable via Empower CDS.
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