Atlantis Columns
Reversed-phase and HILIC HPLC Columns for superior polar compound retention
Polar analytes are difficult to retain using traditional reversed-phase (RP) chromatography as they often elute in the void volume of the column. You can solve this challenge today with Atlantis Columns, which retain and separate polar analytes under reversed phase and HILIC conditions and provide quick, accurate, and reproducible quantification with increased retention and sensitivity.
Available in T3, dC18, and the unbonded HILIC chemistries, the Waters Atlantis Column family provides exceptional performance, versatility, retention, and chromatographic stability for polar compounds and balanced retention for broad analyte mixtures.
Atlantis Columns
Unwanted interactions between analytes and metal ions?
Atlantis Premier Columns with MaxPeak HPS technology mitigate interactions with stainless-steel column hardware.
Specifications
Overview
- Retains and separates small, water-soluble polar organic compounds
- Compatible with 100% aqueous mobile phases
- Improves detection limits, method reproducibility, and robustness by eliminating ion-pairing reagents
- Long column lifetimes using low-pH mobile phases
- Fully scalable in 3, 5, and 10 μm particle sizes
Recommended Use: For the reproducible retention and separation of a broad range of polar and non-polar compounds.
Atlantis Column Characteristics
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Superior retention of polar compounds -
Compatible with 100% aqueous mobile phases
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Exceptional column lifetime at low pH
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Universal reversed-phase column for open access environments
Tri-functionally C18 bonding designed to improve column lifetimes and deliver exceptional reproducible performance for an expansive range of compound polarities.
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Superior retention of polar compounds
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Compatible with 100% aqueous mobile phases
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Reversed-phase columns
Designed to deliver exceptional reproducible performance for both polar and non-polar molecules.
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Excellent for the retention of very polar, basic, water-soluble analytes
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Specifically designed and tested for HILIC separations
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Employ highly volatile (>80% organic) mobile phases
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Hydrophilic Interaction Chromatography (HILIC) silica columns
Ideal for mass spectrometry (MS) response and sensitivity.
Ideal for diverse analyte mixtures
When faced with analyzing several compounds across a broad range of polarity, few options will adequately retain polar species without excessively retaining less polar components. Atlantis T3 Columns are a universal, silica-based, reversed-phase C18 line of HPLC Columns, designed to deliver exceptional performance and give you the ability to retain and separate small, water-soluble polar organic compounds.
Seamless transfer across technology platforms
Based on different particle substrates, Waters Atlantis T3 and HSS T3 Columns enable labs to achieve similar selectivity and retention across HPLC and UPLC technology platforms, enabling ease of method transfer across an organization.
Polar compound retention without ion-pairing reagents
Eliminating ion-pairing reagents improves detection limits, method reproducibility, and robustness, while reducing instrument maintenance from harsh mobile-phase environments. Atlantis HILIC Silica Columns retain very polar, organic molecules that cannot be retained using reversed-phase (RP) methods. Unlike the highly aqueous mobile phases required for polar retention in reversed-phase separations, Atlantis HILIC Silica Columns increase retention using volatile organic-based mobile phases, ideal for mass spectrometry (MS) response and sensitivity.
Scale from analytical to prep with ease
Atlantis T3 and HILIC Silica Prep OBD (Optimum Bed Density) Columns offer analytical column performance in preparative chromatography formats to deliver a variety of advantages, including improved bed stability, higher efficiency, longer column lifetime, and increased loadability.
Stay compliant with smart column options
Ideal for compliant laboratories, smart column options are available for identifying and tracking column information. The eCord is a data recording device which is permanently attached to the column body of 2.x µm particle size (2.1 and 3.0 mm internal diameter) and ACQUITY UPLC columns and is standard for those column offerings. Rather, the eConnect column tag is a permanently attached electronic tag device for HPLC columns (3 µm particles) used with Alliance iS HPLC System that is optional. Data from column devices (eCord device or eConnect column tag) can be accessed using the respective system’s console. These smart-column abilities provide a quick overview of column history and can be utilized as a basic troubleshooting tool. Data residing on these devices are automatically imported by and later searchable via Empower CDS.
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