Glycan Standards and Buffers

Glycan Standards and Buffers

Benchmark and validate your glycan columns with ease

Benchmark and validate your glycan columns with ease

Verify your column performance upon receipt and monitor column condition on a regular basis to ensure optimal performance to save valuable research and development time with Waters Glycan Standards. Run your glycan separations on any LC-MS system with ease and confidence using these specifically formulated, pre-packaged standards. Each Glycan Standard is packaged in Waters Certified Vials, eliminating the need to prepare complex mixtures - providing ease of use while minimizing sample loss and discrepancies.

In addition, Waters offers mobile-phase concentrates and buffers made with high quality raw materials to ensure low level adducts that can complicate LC-MS data analysis. These buffers make it very easy to make complicated, but critical mobile phases for HILIC and mixed-mode separations.

The Glycan Performance Standard is 2-AB labeled Human-like IgG.  Glycan Standards and Buffers

Specifications

Overview

  • Verify the performance of your column upon receipt to ensure consistent batch-to-batch and column-to-column performance
  • Monitor the condition of your columns to extend column lifetime
  • Gain confidence in in your analysis with glycan standards that are quality controlled (QC) per lot to guarantee performance
  • Save time with ready-to-use standards as the Glycan Performance Standard is lyophilized for stability and can be directly solubilized
  • Save time with pre-formulated buffers for easy mobile phase preparation for HILIC and mixed-mode separations

Recommended Use: For the verification of LC-MS instrumentation and column performance for released N-glycan analysis.


Glycan Standards

Glycan Standards

Glycan Performance Standard
  • Intended to be used as a benchmark standard for ACQUITY UPLC BEH Glycan, 1.7 µm, Columns
  • Human IgG based
  • QC verified to contain the components needed to benchmark and evaluate HILIC columns

Labeled standards for 2‑aminobenzamide (2-AB) and RapiFluor-MS


Dextran Calibration Ladder
  • Labeled Dextran Calibration Ladder Standards designed to assist with glycan profiling
  • The workable Glucose Unit (GU) value range is twice as much as any other dextran ladder standards, due to an average molecular weight of the glucose homopolymer being higher (~4,500 Dalton)
  • Offers an improved large glycan retention time assignment

Labeled standards for 2‑anthranilic acid (2-AA), 2‑AB, and RapiFluor-MS


Waters Ammonium Formate Solution
  • A concentrated version of the 50 mM Ammonium formate buffer, it contains 10 mL of 50:50 mM Ammonium formate in a water w/ 3.8% Formic acid matrix
  • This allows the end user to directly pour the concentrate into a 1 L bottle of LCMS grade water for the correct concentration of 50 mM at a pH of 4.4
  • Raw materials are all QC’d to ensure high quality mass spectrometry (MS) friendly reagents that minimize adducts







Resources

Documents

Documents


Support

Support


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