LC Prep AutoPurification System

MS- or UV-directed fraction collection

With the AutoPurification HPLC/UV System, your lab can run samples around the clock with unprecedented versatility and consistency. The AutoPurification HPLC-MS System provides you with the flexibility of using high-throughput parallel runs for selective mass-directed fraction collection from hundreds of samples.

The AutoPurification HPLC-MS System with the ACQUITY QDa Mass Detector or the SQ Detector 2 mass spectrometer is designed for everyday mass-directed purification, so you can collect more of what you want more affordably and easily. With the System Fluidics Organizer, you can easily manage column selection and column regeneration. Achieve full automation and high throughput capabilities with the system's fluidics layout, and leverage enhanced software features and Waters proprietary Optimum Bed Density (OBD) Column technology for maximum efficiency. 

Open access purification

Whether your company manages preparative LC in a centralized lab or provides purification capabilities in an open access environment, easy-to-use systems and software from Waters allow you to run samples with unattended system operation. This improves productivity by enabling chemists to focus on their results rather than on the instrument.

With Waters systems and software, purification is easier to manage because:

• Chemists can walk up to a system, enter their sample, and have results delivered to their desktop, while an administrator monitors the system and manages instrument access with Open Access software
• Fraction collection software provides full-cycle automation, from evaluation and setting collection thresholds to purification to fraction analysis
• Unique method filtering software enables the user to quickly choose a method from the existing library of methods
• Labs can automatically generate gradients without having to manually buffer solvent concentration, and build column clean-up and system flushing into your method
• A solvent monitoring system enables you to use multiple solvent reservoirs with automated warning responses about instrument status and intelligent shut-down available
• A remote system status monitor tracks the sample queue, instrument, and solvent and waste container status online

Automated fraction collection and analysis

Managing Fraction Collection
FractionLynx Application Manager provides full-cycle automation for purification, from evaluating and setting collection thresholds, to collection of the detected fractions, to tracking the samples and fractions, to presentation of the data in an easy-to-view format. The software can trigger collection using a variety of detector signals including UV/Visible, evaporative light scattering (ELS), mass spectrometry (MS), and analog. Other fraction collection options include one-to-one mapping to retain plate format, secondary collection of peaks that are not of primary concern but not classified as waste, and waste collection.

Sample Evaluation
AutoPurify, a feature of the FractionLynx Application Manager, further automates this process by detecting the presence of the target compound and measures its purity to determine the purification process, from initial analytical screening, through purification, to the analysis of the collected fractions. AutoPurify automatically selects a focused preparative gradient based on an analytical screening run, which provides better quality purification and eliminates the need for expert manual invention. Automatic determination of the collection threshold on a per-sample basis removes the interference from drifting baselines or high background signals and increases ease of use. Fractions can be re-injected automatically if required, and information exported.

Scale from analytical UPLC to prep

Waters ultra-performance liquid chromatography (UPLC) technology has been widely accepted by chromatographers because of its improvements over high-performance liquid chromatography (HPLC) in sensitivity, resolution, and speed of separations. Now scientists can use this technology in the sample screening process as a tool to evaluate samples prior to purification as well as a tool for rapid analysis of fractions.

Scaling up from analytical UPLC to preparative HPLC is possible with the use of focused gradients. The efficiency of UPLC can be carried through to purification, offering a substantial increase in throughput and productivity. In a preparative environment, where the compound of interest is being isolated from the other components in the sample, retaining analytical resolution is not as important as isolating and collecting the single compound of interest.

Create the system that best fits your needs

Build a preparative LC system to match your needs, workload, and budget. Choose from a variety of components to configure a system that's right for your lab. Whether your application requires low- or high-pressure mixing, low or high flow rates, manual or automated injection and collection, you choose the level of functionality and capacity that your application requires. And since Waters Purification Systems are upgradable, you don't have to worry about your investment as your workload increases.

When fraction collection is based on UV absorbance, multiple fractions can result, requiring further analysis to determine the fraction of interest. Run samples around the clock with unprecedented versatility and consistency. Add the System Fluidics Organizer to easily manage column selection and column regeneration. FractionLynx Application Manager manages and automates your sample purification process.

Waters offers multiple detection choices, including mass detection:

• Ultraviolet/visible (UV/Vis) absorbance
• Evaporative light scattering (ELS)
• Photodiode array
• Mass spectrometry (MS)
• Analog

Easily manage sample injection and fraction collection with options:

• Manual injection
• Automated injection
• Automated fraction collection
• Automated injection and fraction collection (3767 Sample Manager)

Choose a solvent delivery system that matches your sample's needs: