ACQUITY UPLC M-Class System
Let your LC inlet do the work for your nano- to micro-scale separations
Many analytical scientists would like to increase the sensitivity of their LC-MS analyses to meet the demanding goals of their labs, however, adapting conventional separations instruments to improve sensitivity can cause problems with reproducibility and reliability.
The ACQUITY UPLC M-Class System delivers the performance you expect from nano- to microscale separations with the usability of an analytical-scale UPLC system. Each part of the system was designed and tested to deliver the right flow for the right column. The ACQUITY UPLC M-Class System provides excellent performance with overflow rates from 200 nL/min to 100 µL/min and a range of column inner diameter (I.D.) from 75 µm to 1.0 mm.
ACQUITY UPLC M-Class System
Specifications
|
Maximum operating pressure |
15,000 psi |
|
pH range |
2 to 10 |
|
Operating flow rate range |
200 nL/min to 100 μL/min without flow splitting |
|
Sample flow path |
Non-ferrous |
|
Injection volume |
0.1 µL to 100.0 µL |
|
Sample compartment |
4–40 ˚C |
|
Pump options |
Binary |
|
Column tracking |
None |
|
Column capacity |
Single column, 75 µm to 4.6 mm internal diameter (ID); up to 250 mm in length |
|
Maximum sample capacity |
Up to 21 plates (with Sample Organizer) |
|
Detector options |
PDA Detector TUV Detector Mass Spectrometers |
|
External control |
MassLynx Software |
|
Physical specifications (µBSM, µSM-FL, TVM) |
Width: 34.3 cm (13.5 in.) Height: 69.7 cm(27.4 in.) Depth: 71.2 cm (28.0 in.) |
Overview
- System of choice for sample-limited applications in proteomics and biomarker discovery
- Higher sensitivity for any mass spectrometer (MS)
- Maximum amount of data from any sample
- Less sample preparation volume and cost
- Reduced solvent consumption
- Increased dynamic range
- Flexible configurations for HDX technology, 2D, and UV detection
Recommended Use: Meeting usability, sensitivity, robustness, and reproducibility requirements for nano- to micro-scale UPLC separations.
Features Header
The analysis you’ve always desired for nano- to microscale separations
The ACQUITY UPLC M-Class System delivers the most robust, reliable, and highest quality nano- to microscale UPLC separations to every laboratory performing LC-MS analyses. With unprecedented usability, the ACQUITY UPLC M-Class System enables laboratories to succeed in analyzing compounds that are severely limited in amount or availability, even amid a complex matrix over several orders of magnitude.
Confidently achieve your laboratory goals with high-efficiency ACQUITY UPLC M-Class Columns ranging from 75 µm to 1 mm I.D. by using complementary mass spectrometry to support our full range of column inner diameters or run 300 µm to 1 mm I.D. columns with optical detection. The system also offers a range of flexible configurations for HDX technology, UV detection, and 2D analysis.
Your inlet to MS
The ACQUITY UPLC M-Class System expands the performance of any nano- to micro-scale MS analysis. It increases the linear dynamic range of your analysis, resulting in better MS analyses by data-dependent acquisition (DDA) or MSE. The direct (non split) automatic solvent flow control algorithms of the ACQUITY UPLC M-Class System provide pulse-less flow for increased retention time reproducibility. Sample integrity is maintained due the ACQUITY UPLC M-Class System’s non-reactive surfaces. New drivers for MassLynx Software integrate the system seamlessly into your existing workflows.
Monitor protein conformation with HDX technology
The ACQUITY UPLC M-Class System with HDX Technology can quantify small changes in protein conformation by extending its pressure range to enable a higher efficiency separation. The system allows for manual sample injection and integrates the steps of hydrogen-deuterium exchange (HDX) from sample prep to actual sequence determination, D2 uptake curves, and comparison plots with the interactive, highly innovative DynamX HDX Data Analysis Software. HDX data can be used to make business decisions about drug candidate binding to a protein target, biopharmaceutical product development, protein-protein interactions, and intellectual property.
Expand the dynamic range of your analysis with 2D separations
The ACQUITY UPLC M-Class System with 2D Technology has streamlined 2D-LC separations with a highly intuitive menu-driven method setup, standardized separation chemistries, and intelligent valve operation. Expanding on the use of a novel 2D reversed-phase/reversed-phase (RP/RP) approach, where the first dimension is run at pH 10 and the second at pH 2, grants the ability to robustly increase sample throughput with MS-compatible eluents.
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